CAJ | 학술논문

目的研究NgR1在CPR后大鼠脑组织中的表达.方法采用经皮电刺激致心脏骤停方法,60只SD大鼠分组,按照Uestein模式进行CPR,分别于ROSC(restoration of spontaneous circulation)后1d、3d和7d处死动物,利用免疫荧光及Q-PCR方法分别检测脑组织中NgR1蛋白、NgR1 mRNA的分布和变化.结果 CPR后1d大鼠海马部位NgR1表达明显升高,CPR后7d降至正常.Q-PCR结果同免疫荧光细胞计数结果相似,但各时点NgR1 mRNA表达差异并无统计学意义(P＜0.05).结论 CPR后大鼠脑组织海马NgR1表达呈现一过性升高,为进一步探讨拮抗NgR1对大鼠CPR后脑功能障碍的作用,寻找改善脑复苏预后的新途径奠定基础.
목적 연구NgR1재CPR후대서뇌조직중적표체.방법 채용경피전자격치심장취정방법,60지SD대서분조,안조Uestein모식진행CPR,분별우ROSC(restoration of spontaneous circulation)후1d、3d화7d처사동물,이용면역형광급Q-PCR방법분별검측뇌조직중NgR1단백、NgR1 mRNA적분포화변화.결과 CPR후1d대서해마부위NgR1표체명현승고,CPR후7d강지정상.Q-PCR결과동면역형광세포계수결과상사,단각시점NgR1 mRNA표체차이병무통계학의의(P＜0.05).결론 CPR후대서뇌조직해마NgR1표체정현일과성승고,위진일보탐토길항NgR1대대서CPR후뇌공능장애적작용,심조개선뇌복소예후적신도경전정기출.
Objective To investigate the NgR1 expression in the cerebrum after cardiopulmonary resuscitation (CPR) in rats.Methods Cardiac arrest was induced by alternating current in 60 S-D rats through transcutaneous electrical epicardium stimulation,and CPR was performed with Utstein mode at 6 min after CA.Rats were sacrificed at 1,3 and 7 days after CPR.We performed immunofluorescence with antibodies against NgR1 to map the distribution of NgR1 protein in the hippocampus,while Q-PCR was performed for quantitative analysis of NgR1 mRNA.Results NgR1 expression appeared elevated at 1 d and 3 d after CPR and down to the normal level at 7 d in hippocampus.The expression of NgR1 mRNA in hippocampus and cortical cortex showed the same tendency with the staining outcome.The changes were not significant between each time point compared with.baseline in either hippocampus.But there were significant differences between hippocampus and cortical cortex at each time point after the CPR (P ＜0.05).Conclusions Neuronal NgR1 expression is transient changed during 7 days post-CPR in hippocampus in the rats.