CAJ | 학술논문

为提供非洲菊化学诱变的基础材料以及相应的组培操作技术,采用非洲菊品种‘琳达’的幼嫩花托为外植体进行组织培养研究,外植体最佳消毒时间为流水冲洗1 h,75%的酒精浸泡30 s,0.1%的HgCl2灭菌12 min;诱导产生愈伤组织的最适培养基为：MS＋6-BA 5.0 mg/L＋NAA 0.5 mg/L＋蔗糖3%（w/v）＋琼脂粉7%（w/v）;增殖培养基配方MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L＋蔗糖3%（w/v）＋琼脂粉7%（w/v）为最适配方;生根壮苗培养基以1/2MS＋NAA0.1 mg/L＋蔗糖2%＋琼脂粉7%为最佳。试验显示以花托为外植体较为容易进行表面灭菌,诱导愈伤组织并分化丛生芽,从而快速建立离体快繁体系。
위제공비주국화학유변적기출재료이급상응적조배조작기술,채용비주국품충‘림체’적유눈화탁위외식체진행조직배양연구,외식체최가소독시간위류수충세1 h,75%적주정침포30 s,0.1%적HgCl2멸균12 min;유도산생유상조직적최괄배양기위：MS＋6-BA 5.0 mg/L＋NAA 0.5 mg/L＋자당3%（w/v）＋경지분7%（w/v）;증식배양기배방MS＋6-BA 1.0 mg/L＋NAA 0.1 mg/L＋자당3%（w/v）＋경지분7%（w/v）위최괄배방;생근장묘배양기이1/2MS＋NAA0.1 mg/L＋자당2%＋경지분7%위최가。시험현시이화탁위외식체교위용역진행표면멸균,유도유상조직병분화총생아,종이쾌속건립리체쾌번체계。
To provide the basis of the materials for Gerbera’s chemical inducement;and the corresponding set of technique.Using gerbera varieties young receptacle of‘Linda’as explants to tissue culture.the best sterilization time on explants were washed for one hour and sterilized with 75% ethyl alcohol for 30 s,0.1% HgCl2 for 12 minutes,the calluses introduction medium for in vitro culture was MS（murashige and skoog） with 6-BA（5.0 mg/L）＋ NAA（0.5 mg/L）＋ sucrose（3%）＋ agar（7%）,the multiplication medium was MS with 6-BA（1.0 mg/L）＋NAA（0.1mg/L）＋ sucrose（3%）＋agar（7%）,the rooting medium was 1/2MS with NAA（0.1 mg/L）＋ sucrose（2%）＋agar（7%）.From the experiments,it was evident that young receptacle as explants was more easy to surface sterilization,callus induction and multiple shoot clumps,then established rapid propagation system of Gerbera.