CAJ | 학술논문

本研究对福建长乐、古雷、晋江海区裸体方格星虫自然群体91个个体的线粒体COI基因进行序列测定,并与厦门、北海、三亚3个地理群体15个个体的线粒体COI基因序列进行了比较分析。在106个个体中共发现193个核苷酸多态位点和52个单倍型,长乐、古雷、晋江海区3个地理群体中单倍型多样性指数（h）在0.626到0.972之间,核苷酸多样性（π）在0.00155到0.01325之间,表现出较高的遗传多样性。分子方差分析（AMOVA）显示1.97%的变异存在于群体内,而群体间的遗传变异为98.03%,群体间变异是总变异的主要来源。构建的单倍型系统关系树显示单倍型聚合为A、B、C、D 4个不同的具有高支持率的分支,表明我国沿海至少存在4个线粒体具显著差异的类群,并可能存在隐蔽种。单倍型最小拓展网络图揭示裸体方格星虫类群A存在福建沿海和北部湾两个明显的分支,是否可提升为亚种有待进一步考证。研究结果为中国沿海裸体方格星虫种质资源保护、人工苗种放养及遗传育种工作的开展提供了依据。
본연구대복건장악、고뢰、진강해구라체방격성충자연군체91개개체적선립체COI기인진행서렬측정,병여하문、북해、삼아3개지리군체15개개체적선립체COI기인서렬진행료비교분석。재106개개체중공발현193개핵감산다태위점화52개단배형,장악、고뢰、진강해구3개지리군체중단배형다양성지수（h）재0.626도0.972지간,핵감산다양성（π）재0.00155도0.01325지간,표현출교고적유전다양성。분자방차분석（AMOVA）현시1.97%적변이존재우군체내,이군체간적유전변이위98.03%,군체간변이시총변이적주요래원。구건적단배형계통관계수현시단배형취합위A、B、C、D 4개불동적구유고지지솔적분지,표명아국연해지소존재4개선립체구현저차이적류군,병가능존재은폐충。단배형최소탁전망락도게시라체방격성충류군A존재복건연해화북부만량개명현적분지,시부가제승위아충유대진일보고증。연구결과위중국연해라체방격성충충질자원보호、인공묘충방양급유전육충공작적개전제공료의거。
Genetic structure and population differentiation in wild populations of Sipunculus nudus were researched in this paper.91 samples collected from Changle、Gulei、Jinjiang in Fujian province and 15 from Xiamen、Beihai、Sanya were studied based on sequence analyses of the mitochondrial cytochrome oxidase I（mtDNA COI） gene segment.193 nucleotide polymorphisms and 52 haplotypes were found in total 106 samples.Among three Fujian geographical groups,gene diversities（π） ranged from 0.00155 to 0.01325,and the nucleotide diversities（h） from 0.626 to 0.972,showed that Sipunculus nudus had high genetic diversity.AMOVA analysis disclosed that variation among populations accounted for 98.03% of total variation,revealed that variations occurred between populations were the main source of the total variation.Neighbor-joining tree based on mtDNA COI sequence showed A、B、C and D,the four different branches with high support value.The four distinct clades among south China coastal waters revealed underestimate cryptic diversity within Sipunculus nudus.Minimum spanning network from all haplotypes revealed two distinct branches as Fujian and the northern Gulf in groups A,which might be approached to subspecies level upon further research.This study would be useful for the resources protection,artificial seed stocking and genetic breeding of Sipunculus nudus.