CAJ | 학술논문

研究通过骨髓瘤细胞SP2／O与经金黄色葡萄球菌（S．aureus）Trap蛋白免疫的小鼠脾细胞融合，并进一步筛选种单克隆化，获得了11株稳定分泌抗TraP蛋白的单克隆抗体（McAb）杂交瘤细胞株，其中8株McAb（2A1、3A6、381、184、2C5、4C7、5C3和2D8）亚类为IgGl，3株McAb（2A7、3A1和1c4）亚类为IgM，轻链均为K链。8株IgGl型McAb的小鼠腹水的抗体效价均达到1：128000，交叉实验结果显示这8株McAb不与S．aureus的ClfA、ClfB、Cna、FnbPA和IsdB蛋白以及链球菌的GapC蛋白反应，具有良好的特异性，Western—blotting结果显示这8株McAb识别的都是TraP的线性表位。
연구통과골수류세포SP2／O여경금황색포도구균（S．aureus）Trap단백면역적소서비세포융합，병진일보사선충단극륭화，획득료11주은정분비항TraP단백적단극륭항체（McAb）잡교류세포주，기중8주McAb（2A1、3A6、381、184、2C5、4C7、5C3화2D8）아류위IgGl，3주McAb（2A7、3A1화1c4）아류위IgM，경련균위K련。8주IgGl형McAb적소서복수적항체효개균체도1：128000，교차실험결과현시저8주McAb불여S．aureus적ClfA、ClfB、Cna、FnbPA화IsdB단백이급련구균적GapC단백반응，구유량호적특이성，Western—blotting결과현시저8주McAb식별적도시TraP적선성표위。
Eleven antibody-secreting hybridomas were produced in vitro by fusing myeloma cells line SP2/0 with spleen cells from a BALB/c mice immunized with Staphylococcus aureus Target of RNAIII-activiting protein （TraP）. Eight （2A1,3A6,3B1,1B4,2C5, 4C7,5C3 and 2D8）of these monoelonal anti-TraP antibodies were characterized as subtype IgG1 and K chain, and the antibody titers of McAb produced by these eight hybridomas in ascites were 1：128 000. These McAbs specific for TraP were identified by an indirect ELISA against protein ClfA,ClfB,Cna,FnbPA and IsdB from Staphylococcus aureus and GapC from Streptococcus. Results of western-blotting suggested that these McAbs were the lined epitopes of TraP.