CAJ | 학술논문

以干酪乳杆菌作为呈递抗原活载体，表达新城疫病毒保护性抗原HN蛋白。重组于酪乳杆菌表达外源蛋白后，经SDS-PAGE、Westernblot及间接免疫荧光分析，表明目的蛋白表达并展示到乳酸菌表面。将重组菌及空质粒菌株分别滴鼻、点眼免疫雏鸡，于不同时间检测血清样品中特异性IgG；于三免后不同时间采集雏鸡的泪液、气管洗液、胆汁和肠洗液样品，采用间接ELISA方法检测样品的特异性sIgA；用MTY法检测免疫鸡脾淋巴细胞增殖情况，结果显示重组干酪乳杆菌表达系统能刺激动物黏膜免疫反应和系统免疫反应。
이간락유간균작위정체항원활재체，표체신성역병독보호성항원HN단백。중조우락유간균표체외원단백후，경SDS-PAGE、Westernblot급간접면역형광분석，표명목적단백표체병전시도유산균표면。장중조균급공질립균주분별적비、점안면역추계，우불동시간검측혈청양품중특이성IgG；우삼면후불동시간채집추계적루액、기관세액、담즙화장세액양품，채용간접ELISA방법검측양품적특이성sIgA；용MTY법검측면역계비림파세포증식정황，결과현시중조간락유간균표체계통능자격동물점막면역반응화계통면역반응。
Neweasfle disease virus （NDV） HN antigen was expressed by the recombinant Lactobacillus casei. The HN protein was surface-displayed on the L.casei verified by Immunofluorescence Microscopy, SDS-PAGE and Western blot. Intranasal and eye dropping immunized chicks was performed with the recombinant strain L. casei harboring pLA-HN or pLA. Specific anti-HN IgG antibody in the serum and specific anti-HN secret immunoglobulin A（sIgA） antibody in the lung and intestine fluid, tear and bile of chicken were detected by indirect ELISA.T-cell proliferation responses were investigated by MTT test. The results demonstrated that the recombinant L.casei could induce the mucosal and systemic immunity.