农学学报
農學學報
농학학보
Chinese Countryside Well-off Technology
2012年
2期
42-45,78
,共5页
王伟英%邹晖%林江波%戴艺民
王偉英%鄒暉%林江波%戴藝民
왕위영%추휘%림강파%대예민
彩色马蹄莲%组织培养%愈伤组织%生根%快繁
綵色馬蹄蓮%組織培養%愈傷組織%生根%快繁
채색마제련%조직배양%유상조직%생근%쾌번
Zantedeschia hybrida%Tissue Culture%Callus%Rooting%Rapid Propagation
以彩色马蹄莲‘高原’块茎为材料研究其组培快繁技术,筛选适合彩色马蹄莲快繁的培养基。结果表明：在初代试验中对块茎的消毒以0.1%HgCl2处理15 min为宜;MS＋0.5 mg/L 6-BA＋0.1~0.2 mg/LNAA为适宜的愈伤组织诱导培养基;MS＋2.0 mg/L 6-BA＋0.2 mg/L NAA能使愈伤组织分化出状态良好的丛生芽并继续增殖;在生根培养基1/2MS＋0.2 mg/L NAA＋10%香蕉上生根率100%,试管苗生长健壮,根系粗壮发达。试验为彩色马蹄莲的快繁体系及规模化生产提供参考依据。
以綵色馬蹄蓮‘高原’塊莖為材料研究其組培快繁技術,篩選適閤綵色馬蹄蓮快繁的培養基。結果錶明：在初代試驗中對塊莖的消毒以0.1%HgCl2處理15 min為宜;MS＋0.5 mg/L 6-BA＋0.1~0.2 mg/LNAA為適宜的愈傷組織誘導培養基;MS＋2.0 mg/L 6-BA＋0.2 mg/L NAA能使愈傷組織分化齣狀態良好的叢生芽併繼續增殖;在生根培養基1/2MS＋0.2 mg/L NAA＋10%香蕉上生根率100%,試管苗生長健壯,根繫粗壯髮達。試驗為綵色馬蹄蓮的快繁體繫及規模化生產提供參攷依據。
이채색마제련‘고원’괴경위재료연구기조배쾌번기술,사선괄합채색마제련쾌번적배양기。결과표명：재초대시험중대괴경적소독이0.1%HgCl2처리15 min위의;MS＋0.5 mg/L 6-BA＋0.1~0.2 mg/LNAA위괄의적유상조직유도배양기;MS＋2.0 mg/L 6-BA＋0.2 mg/L NAA능사유상조직분화출상태량호적총생아병계속증식;재생근배양기1/2MS＋0.2 mg/L NAA＋10%향초상생근솔100%,시관묘생장건장,근계조장발체。시험위채색마제련적쾌번체계급규모화생산제공삼고의거。
The bulbs of Zantedeschia hybrida.‘Gaoyuan’were used as explants to study the tissue culture and rapid propagation,and to screen the best culture medium for rapid propagation.The best disinfectant time by 0.1% HgCl2 was 15 minutes.The best medium for callus induction was MS ＋ 0.5 mg/L 6-BA ＋ 0.1-0.2 mg/L NAA.Callus grew into cluster buds and the buds grew well in the medium of MS＋2.0 mg/L 6-BA＋0.2 mg/L NAA.In the medium of 1/2MS ＋ 0.2 mg/L NAA ＋ 10% banana,the plantlets in vitro had the rooting rate of 100%,and their root system grew vigourly.
