CAJ | 학술논문

利用双向凝胶电泳和基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）技术,研究与人肺永生化支气管上皮细胞（BEP2D细胞）辐照相关的差异蛋白质,寻找辐射致肺癌早期诊断和治疗的新指标。结果表明,提取样品蛋白进行二维凝胶电泳,找到差异表达蛋白质点约18个,质谱鉴定获得10张肽质指纹图,经数据库搜索后初步鉴定差异表达的蛋白质有7种,即亲环素A、肌球蛋白轻链2、细胞角蛋白9、α-烯醇酶、磷酸丙糖异构酶1、MKRN1、c-myc启动子结合蛋白1。该7种蛋白质与辐射诱导肺癌的发生、发展过程密切相关。
이용쌍향응효전영화기질보조격광해석전리비행시간질보（MALDI-TOF-MS）기술,연구여인폐영생화지기관상피세포（BEP2D세포）복조상관적차이단백질,심조복사치폐암조기진단화치료적신지표。결과표명,제취양품단백진행이유응효전영,조도차이표체단백질점약18개,질보감정획득10장태질지문도,경수거고수색후초보감정차이표체적단백질유7충,즉친배소A、기구단백경련2、세포각단백9、α-희순매、린산병당이구매1、MKRN1、c-myc계동자결합단백1。해7충단백질여복사유도폐암적발생、발전과정밀절상관。
The samples of the BEP2D cell radiated with 0、1.5 and 3.0 Gy γ rays were cultivated and collected,and the total protein were separated with the two-dimensional gel electrophoresis.The peptide mass fingerprint were obtained using matrix-assisted laser desorption ionization time of flight mass spectrometry（MALDI-TOF-MS）for differentially expressed proteins.10 peptide mass fingerprinting are reveal in the mass spectra identification,8 of them are approved in the database,and 1 are duplicated;2 are null. There are 7 points identified in the initial testsby Mascot software in the NCBInr database,such as Cyclophilin A,Myosin light chain 2,Cytokeratin 9,α-enolase,Triosephosphate isomerase 1,Makorin ring finger protein 1,c-myc promoter-binding protein 1.these 7 kinds of proteins probablely are the special proteins related to the lung cancer induced by irradiation,which might be worth of further study of the functions of proteins.