CAJ | 학술논문

目的建立适用于中国人群的MDS诊断流式细胞学评分系统,以提高MDS早期诊断的准确性.方法运用多色流式细胞术检测确诊MDS组和对照组CD_(34)~+细胞和各分化阶段细胞免疫表型的异常,建立MDS诊断的流式细胞学评分系统;应用该评分系统对37例临术床疑似MDS患者进行评分.结果筛选出12个对MDS诊断有贡献的干细胞或分化阶段细胞抗原表达异常作为MDS流式细胞学诊断的积分指标,根据其在MDS中出现的特异性分别给予1分和0.5分,建立了MDS诊断的流式细胞学评分系统;37例试验组中确诊为MDS的患者评分均＞2分,确诊为非MDS的患者评分均＜5分,当以积3分为截断值时,诊断MDS的敏感度和特异度可分别达到94.1%和80.1%.结论建立流式细胞学评分系统能有效地辅助MDS的早期诊断和准确诊断.
목적 건립괄용우중국인군적MDS진단류식세포학평분계통,이제고MDS조기진단적준학성.방법 운용다색류식세포술검측학진MDS조화대조조CD_(34)~+세포화각분화계단세포면역표형적이상,건립MDS진단적류식세포학평분계통;응용해평분계통대37례림술상의사MDS환자진행평분.결과 사선출12개대MDS진단유공헌적간세포혹분화계단세포항원표체이상작위MDS류식세포학진단적적분지표,근거기재MDS중출현적특이성분별급여1분화0.5분,건립료MDS진단적류식세포학평분계통;37례시험조중학진위MDS적환자평분균＞2분,학진위비MDS적환자평분균＜5분,당이적3분위절단치시,진단MDS적민감도화특이도가분별체도94.1%화80.1%.결론 건립류식세포학평분계통능유효지보조MDS적조기진단화준학진단.
Objective To develop a flow cytometric scoring system that is suitable to Chinese population for the early and accurate diagnosis of MDS.Methods Bone marrow from MDS patient and control group were analyzed using 4-colour flow cytometry,and data were collected including immunophenotype of CD_(34)~+ blasts and maturing cells of myeloid lineage.A flow cytometric scoring system were established according to the result of Logistic statistical analysis.The second group of 37 patients who were suspected to have MDS were scored according to the scoring system.Results 12 parameters showed to be contributive to diagnosis of MDS,and were assigned 1 or 0.5 scores separately according to their specificity in MDS population to set up a flow eytometrie scoring system.The scores in the diagnosed MDS patients of the second group were all greater than 2,while in the non-MDS patients the scores were all less than 5.A score of 3 allowed for a sensitivity of 94.1% with specificity 80.1% in MDS diagnosis.Conclusion Flow cytometric scoring system can provide useful information in early and accurate diagnosis of MDS.