江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2014年
1期
174-180
,共7页
阮国荣%何晓芳%黄晶%肖石军%陈金雄%李军山%刘亚轩%孙耀华%陈福珍%林国忠
阮國榮%何曉芳%黃晶%肖石軍%陳金雄%李軍山%劉亞軒%孫耀華%陳福珍%林國忠
원국영%하효방%황정%초석군%진금웅%리군산%류아헌%손요화%진복진%림국충
杜洛克%MUC13%选育
杜洛剋%MUC13%選育
두락극%MUC13%선육
Duroc%MUC13%breeding
MUC13是控制仔猪大肠杆菌F4ac易感或抗性的主效基因。本研究利用分子育种与常规育种技术、闭锁继代与开放选育相结合的综合方法，对250头杜洛克育种核心群实施连续3个世代的基因检测和性能测定，筛选出MUC13抗性纯合基因型种群。经测试，在相同饲养管理条件下，哺乳期的同胎次20窝育种群母猪与20窝扩繁群母猪相比，断奶前仔猪腹泻发生率分别为8．7％和18．4％（P＜0．01），但断奶成活率两组间差异未达到显著水平（ P＞0．05）；对育种群3个世代的测定选育结果分析发现，第一和第三世代间达100 kg体质量日龄差异不显著（P＞0．05）、但活体背膘厚相比减少1．46 mm（P＜0．05），同时还发现，3个世代中MUC13不同基因型个体间生长速度和活体背膘差异均不显著（ P＞0．05）。综上说明，MUC13基因抗性纯合有利于防止仔猪腹泻的发生并能有效降低活体背膘，但对生长发育没有明显影响。
MUC13是控製仔豬大腸桿菌F4ac易感或抗性的主效基因。本研究利用分子育種與常規育種技術、閉鎖繼代與開放選育相結閤的綜閤方法，對250頭杜洛剋育種覈心群實施連續3箇世代的基因檢測和性能測定，篩選齣MUC13抗性純閤基因型種群。經測試，在相同飼養管理條件下，哺乳期的同胎次20窩育種群母豬與20窩擴繁群母豬相比，斷奶前仔豬腹瀉髮生率分彆為8．7％和18．4％（P＜0．01），但斷奶成活率兩組間差異未達到顯著水平（ P＞0．05）；對育種群3箇世代的測定選育結果分析髮現，第一和第三世代間達100 kg體質量日齡差異不顯著（P＞0．05）、但活體揹膘厚相比減少1．46 mm（P＜0．05），同時還髮現，3箇世代中MUC13不同基因型箇體間生長速度和活體揹膘差異均不顯著（ P＞0．05）。綜上說明，MUC13基因抗性純閤有利于防止仔豬腹瀉的髮生併能有效降低活體揹膘，但對生長髮育沒有明顯影響。
MUC13시공제자저대장간균F4ac역감혹항성적주효기인。본연구이용분자육충여상규육충기술、폐쇄계대여개방선육상결합적종합방법，대250두두락극육충핵심군실시련속3개세대적기인검측화성능측정，사선출MUC13항성순합기인형충군。경측시，재상동사양관리조건하，포유기적동태차20와육충군모저여20와확번군모저상비，단내전자저복사발생솔분별위8．7％화18．4％（P＜0．01），단단내성활솔량조간차이미체도현저수평（ P＞0．05）；대육충군3개세대적측정선육결과분석발현，제일화제삼세대간체100 kg체질량일령차이불현저（P＞0．05）、단활체배표후상비감소1．46 mm（P＜0．05），동시환발현，3개세대중MUC13불동기인형개체간생장속도화활체배표차이균불현저（ P＞0．05）。종상설명，MUC13기인항성순합유리우방지자저복사적발생병능유효강저활체배표，단대생장발육몰유명현영향。
MUC13 is a major gene to control ETEC F4ac adhesion that causes diarrhea in piglets .In the present study ,the MUC13 resistant allele was determined and its performance traits were recorded in a Duroc nucleus population of 250 individuals through three successive generations using molecular breeding and con-ventional breeding techniques .After the three-generation selection ,20 litters of breeding sows and 20 litters ofmultiplier sows at the same litter during lactation were observed under the same feeding and management con-ditions in the experiment.As a result,the diarrhea incidence rate was 8.7%and 18.4%in the breeding sows and propagation sows before weaning ,respectively.Of note,this difference was significant (P<0.01) in two groups while no significant difference was found in survival rate between these two groups when weaned .Fur-thermore,no significant difference in the date when the weight was up to 100 kg after 3 generation’s measure-ment and selection was found between the first generation and third generation in the breeding population .On the contrary,live backfat thickness was reduced by 1.46 mm and showed a significant difference between the two generations (P<0.05).In addition,no significant differences were found in growth rate and live backfat thickness across the different individuals of genotype .In conclusion ,the MUC13-resistant allele can confer re-sistance to diarrhea in piglets and reduce live backfat thickness in adult pigs whereas has no significant effect on growth and development .
