中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
23期
111-112
,共2页
环孢素A%高效液相色谱%血药浓度监测
環孢素A%高效液相色譜%血藥濃度鑑測
배포소A%고효액상색보%혈약농도감측
Cyclosporine A%HPLC%Blood concentration determination
目的：建立环孢素A血药浓度的HPLC测定方法。方法：采用反相HPLC的方法，血样经乙醚萃取，正己烷洗脱等处理后测定。采用DiamonsilTM C18色谱柱，流动相为乙腈-水（74：26，V/V），流速为1 ml/min，柱温为70℃，检测波长为210 nm。结果：环孢素A血药浓度在50~1000 ng/ml范围内线性关系良好，R2=0.9997，低中高三种浓度的提取回收率为70.01%~75.00%，方法回收率为98.93%~103.08%，日内精密度RSD为0.84%~4.76%，日间精密度RSD为5.38%~9.87%。结论：该方法快速灵敏准确，可用于环孢素A血药浓度的监测。
目的：建立環孢素A血藥濃度的HPLC測定方法。方法：採用反相HPLC的方法，血樣經乙醚萃取，正己烷洗脫等處理後測定。採用DiamonsilTM C18色譜柱，流動相為乙腈-水（74：26，V/V），流速為1 ml/min，柱溫為70℃，檢測波長為210 nm。結果：環孢素A血藥濃度在50~1000 ng/ml範圍內線性關繫良好，R2=0.9997，低中高三種濃度的提取迴收率為70.01%~75.00%，方法迴收率為98.93%~103.08%，日內精密度RSD為0.84%~4.76%，日間精密度RSD為5.38%~9.87%。結論：該方法快速靈敏準確，可用于環孢素A血藥濃度的鑑測。
목적：건립배포소A혈약농도적HPLC측정방법。방법：채용반상HPLC적방법，혈양경을미췌취，정기완세탈등처리후측정。채용DiamonsilTM C18색보주，류동상위을정-수（74：26，V/V），류속위1 ml/min，주온위70℃，검측파장위210 nm。결과：배포소A혈약농도재50~1000 ng/ml범위내선성관계량호，R2=0.9997，저중고삼충농도적제취회수솔위70.01%~75.00%，방법회수솔위98.93%~103.08%，일내정밀도RSD위0.84%~4.76%，일간정밀도RSD위5.38%~9.87%。결론：해방법쾌속령민준학，가용우배포소A혈약농도적감측。
Objective:To establish a HPLC method of detecting cyclosporine A concentration in blood. Method:Cyclosporine A was extracted from whole blood with diethyl ether,and the endogenous interference was removed from blood sample with n-hexane. HPLC was adopted including with DiamonsilTM C18 column and the 70℃temperature.The mobile phase was consisted of acetonitrile-water(74:26,V/V)at the flow rate of 1 ml/min with the detection wavelength at 210 nm. Result:The linear range of cyclosporine A was good(R2=0.9997).The extraction recovery was 70.01%~75.00%and the method recovery was 98.93%~103.08%.The intra-day precision RSD was 0.84%~4.76%,and the inter-day precision RSD was 5.38%~9.87%. Conclusion:The established method is rapid,sensitive and accurate,therefore,it is suitable for the drug monitoring of cyclosporine A in the clinic.
