CAJ | 학술논문

　　目的探讨兰索拉唑（Lansoprazole,LPZ)对幽门螺杆菌（Helicobacter pylori，Hp）感染的胃上皮细胞的体外抗炎效应。方法体外培养胃上皮细胞系AGS，加入LPZ和培养的Hp感染不同时间。分别采用Real time PCR和Western blot检测AGS细胞中环氧化酶2（cyclooxygenase-2，COX-2） mRNA和蛋白的表达，ELISA分别检测PGE 2的产生。结果 LPZ处理后，COX-2表达显著降低，同时也能降低PGE 2的产生。结论 LPZ能抑制COX-2的表达及PGE 2的产生，提示LPZ可预防Hp感染的胃上皮细胞炎症。
　　목적탐토란색랍서（Lansoprazole,LPZ)대유문라간균（Helicobacter pylori，Hp）감염적위상피세포적체외항염효응。방법체외배양위상피세포계AGS，가입LPZ화배양적Hp감염불동시간。분별채용Real time PCR화Western blot검측AGS세포중배양화매2（cyclooxygenase-2，COX-2） mRNA화단백적표체，ELISA분별검측PGE 2적산생。결과 LPZ처리후，COX-2표체현저강저，동시야능강저PGE 2적산생。결론 LPZ능억제COX-2적표체급PGE 2적산생，제시LPZ가예방Hp감염적위상피세포염증。
Objective To investigat the anti-inlfammatory effects of Lansoprazole (LPZ) in H. pylori-induced gastric epithelial cells. Methods Gastric epithelial cell line AGS was cultured in vitro, and was incubated with LPZ and H. pylori for various times. Expression of cyclooxygenase-2 (COX-2) mRNA and protein were detected by real-time PCR and Western blot, respectively;Production of PGE 2 was detected by ELISA. Results LPZ treatments signiifcantly decreased the COX-2 mRNA and protein expression, and the level of PGE 2 was also inhibit, as demonstrated by ELISA. Conclusion LPZ effectively inhibited COX-2 expression and PGE 2 production, The compound shows great potential as a candidate agent for the inhibition of H. pylori-induced extensive gastric epithelial cell inlfammation.