中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
32期
5877-5882
,共6页
干细胞%干细胞综述%脐血%间充质干细胞%多系分化%低免疫原性%缺损%修复%移植物抗宿主病%造血支持%分离%优化培养
榦細胞%榦細胞綜述%臍血%間充質榦細胞%多繫分化%低免疫原性%缺損%脩複%移植物抗宿主病%造血支持%分離%優化培養
간세포%간세포종술%제혈%간충질간세포%다계분화%저면역원성%결손%수복%이식물항숙주병%조혈지지%분리%우화배양
stem cel s%stem cel review%umbilical cord blood%mesenchymal stem cel s%multi-lineage differentiation%low immunogenicity%defect%repairing%graft versus host diseases%hematopoietic support%isolation%culture optimization
背景：脐血源间充质干细胞是来源于发育早期中胚层的一类多能干细胞，因其具有多向分化潜能的特点而日益受到关注。目的：综述脐血来源间充质干细胞的诱导分化潜能。方法：应用计算机检索 Pubmed数据库中1999年1月至2012年12月关于脐血源间充质干细胞分化潜能的文章，在标题和中以“umblical cord blood，mesenchymal stem cel s，potential，differentiation”为检索词进行检索。最终选择关于脐血源间充质干细胞分化潜能的52篇文献进行综述。结果与结论：虽然很多实验已经证实人脐血间充质干细胞可以成功向多种细胞系分化，但对其了解程度尚浅。如果能掌握脐血间充质干细胞分化潜能的特点，那么很可能用它来修复许多骨缺损、心肌缺损等。目前研究正处于起步阶段，在分离纯化技术、分化方向的调控、体外扩增、免疫原性等方面尚有待进一步深入研究。
揹景：臍血源間充質榦細胞是來源于髮育早期中胚層的一類多能榦細胞，因其具有多嚮分化潛能的特點而日益受到關註。目的：綜述臍血來源間充質榦細胞的誘導分化潛能。方法：應用計算機檢索 Pubmed數據庫中1999年1月至2012年12月關于臍血源間充質榦細胞分化潛能的文章，在標題和中以“umblical cord blood，mesenchymal stem cel s，potential，differentiation”為檢索詞進行檢索。最終選擇關于臍血源間充質榦細胞分化潛能的52篇文獻進行綜述。結果與結論：雖然很多實驗已經證實人臍血間充質榦細胞可以成功嚮多種細胞繫分化，但對其瞭解程度尚淺。如果能掌握臍血間充質榦細胞分化潛能的特點，那麽很可能用它來脩複許多骨缺損、心肌缺損等。目前研究正處于起步階段，在分離純化技術、分化方嚮的調控、體外擴增、免疫原性等方麵尚有待進一步深入研究。
배경：제혈원간충질간세포시래원우발육조기중배층적일류다능간세포，인기구유다향분화잠능적특점이일익수도관주。목적：종술제혈래원간충질간세포적유도분화잠능。방법：응용계산궤검색 Pubmed수거고중1999년1월지2012년12월관우제혈원간충질간세포분화잠능적문장，재표제화중이“umblical cord blood，mesenchymal stem cel s，potential，differentiation”위검색사진행검색。최종선택관우제혈원간충질간세포분화잠능적52편문헌진행종술。결과여결론：수연흔다실험이경증실인제혈간충질간세포가이성공향다충세포계분화，단대기료해정도상천。여과능장악제혈간충질간세포분화잠능적특점，나요흔가능용타래수복허다골결손、심기결손등。목전연구정처우기보계단，재분리순화기술、분화방향적조공、체외확증、면역원성등방면상유대진일보심입연구。
BACKGROUND:Umbilical cord blood-derived mesenchymal stem cel s are multipotential stem cel s in the mesoderm in early development stage, and have been paid great attention due to its properties of multi-directional differentiation. OBJECTIVE:To summarize the potential of induced differentiation of umbilical cord blood-derived mesenchymal stem cel s. METHODS:We retrieved PubMed Database for articles concerning the differentiation potential of umbilical cord blood-derived mesenchymal stem cel s published from January 1999 to December 2012. In titles and abstracts, the key words were“umblical cord blood, mesenchymal stem cel s, potential, differentiation”. Total y, 52 articles addressing the differentiation potential of umbilical cord blood-derived mesenchymal stem cel s were reviewed. RESULTS AND CONCLUSION:Numerous studies have confirmed that human umbilical cord blood-derived mesenchymal stem cel s can successful y differentiate into multiple kinds of cel lines, but their understanding remains minor. If we can master the characteristics of the differentiation potential of umbilical cord blood-derived mesenchymal stem cel s, it would be used to repair bone and myocardium detects. Present studies remain in a starting stage. Isolation and purification, regulation of differentiation direction, in vitro amplification and immunogenicity require further investigations.
