CAJ | 학술논문

目的：探讨雌孕激素受体（ER、PR）及同源框基因-11（HOXA-11）在人子宫内膜种植窗口期的表达。方法：采用免疫组化和RT-PCR方法检测ER、PR及HOXA-11在拟行IVF-ET患者排卵后子宫内膜种植窗口期的表达。按IVF-ET后是否妊娠将153例因输卵管因素不孕的患者分为未妊娠组（n＝86）和妊娠组（n＝67）。结果：免疫组化结果显示PR和HOXA-11在妊娠组腺上皮细胞中的表达均低于未妊娠组（ P均＜0．001），在间质细胞中的表达均高于未妊娠组（P均＜0．001）。 RT-PCR 结果显示妊娠组子宫内膜 ER、PR 及 HOXA-11 mRNA 的相对表达量均高于未妊娠组（P均＜0．05）。 P水平与腺上皮、间质PR及腺上皮HOXA-11蛋白表达有关（P均＜0．05）。结论：种植窗口期内膜腺上皮PR、HOXA-11表达下调和间质细胞PR、HOXA-11表达上调的失败可能是导致子宫内膜容受性下降的原因之一。
목적：탐토자잉격소수체（ER、PR）급동원광기인-11（HOXA-11）재인자궁내막충식창구기적표체。방법：채용면역조화화RT-PCR방법검측ER、PR급HOXA-11재의행IVF-ET환자배란후자궁내막충식창구기적표체。안IVF-ET후시부임신장153례인수란관인소불잉적환자분위미임신조（n＝86）화임신조（n＝67）。결과：면역조화결과현시PR화HOXA-11재임신조선상피세포중적표체균저우미임신조（ P균＜0．001），재간질세포중적표체균고우미임신조（P균＜0．001）。 RT-PCR 결과현시임신조자궁내막 ER、PR 급 HOXA-11 mRNA 적상대표체량균고우미임신조（P균＜0．05）。 P수평여선상피、간질PR급선상피HOXA-11단백표체유관（P균＜0．05）。결론：충식창구기내막선상피PR、HOXA-11표체하조화간질세포PR、HOXA-11표체상조적실패가능시도치자궁내막용수성하강적원인지일。
Aim:To investigate the expressions of ER , PR and HOXA-11 in human endometrium during window peri-od.Methods:Using immunohistochemical staining and RT-PCR the expressions of ER , PR and HOXA-11 in human endo-metrium during the window period were detected .According to pregnancy or not after IVF-ET, 78 cases of infertility pa-tients with tubal factors were divided into pregnant group ( n=86 ) and non-pregnant group ( n=67 ) .Results: The ex-pressions of PR and HOXA-11 in the glandular epithelium of the pregnant group were significantly lower than those of the non-pregnant group(P<0.001), while in the stromal cells were significantly higher than those of the non-pregnant group (P<0.001).The expressions of ER,PR and HOXA-11 mRNA of the pregnant group were higher than those of the non-pregnant group(P<0.05).There was correlation between level of P and PR,HOXA-11 in glandular epithelial(P<0.05). Conclusion:The down-regulated expressions of PR and HOXA-11 in glandular epithelial cells and the up-regulated expres-sions of PR and HOXA-11 in mesenchymal cells may be the reasons which lead to the decrease of endometrial receptivity .