CAJ | 학술논문

目的建立去卵巢大鼠模型,观察性激素水平改变对泪腺中IL-17A以及IL-1β、IL-6、TNF-α等炎症因子表达的影响.方法实验研究.健康雌性SD大鼠20只随机分为2组,实验组(10只)摘除大鼠双侧卵巢,对照组(10只)为未摘除卵巢的模拟手术组.术前及术后第1个月、第2个月、第3个月时2组均行泪液分泌试验(SIT)、角膜荧光染色检查进行眼表评估.于第3个月时放射免疫分析法分别检测2组大鼠血清雌二醇、睾酮含量,HE染色观察泪腺上皮细胞形态,免疫组化染色法、Western Blot法检测2组泪腺中IL-17A、IL-1β、IL-6、TNF-α的表达.2组性激素质量浓度比较、炎症因子A值表达比较均应用独立样本t检验.2组各时间点的SIT结果比较采用重复测量资料两因素方差分析.结果实验组大鼠去卵巢后第3个月时,体内雌二醇浓度低于对照组(t=-35.37,P＜0.01),睾酮浓度低于对照组(t=-12.13,P＜0.01).2组各时期SIT检查未见泪液分泌减少、均未见角膜荧光染色.HE染色见实验组泪腺腺泡萎缩,排列紊乱,胞质内酶原颗粒明显减少.泪腺IL-17A表达量2组比较实验组高于对照组(免疫组化法:t=7.56,P＜0.01;Western Blot法:t=20.90,P＜0.01).余各因子表达量实验组也均高于对照组(免疫组化法:IL-1β:t=13.71,P＜0.01;IL-6:t=13.92,P＜0.01;TNF-α:t=6.11,P＜0.01.Western Blot法:IL-1β:t=16.93,P＜0.01;IL-6:t=12.46,P＜0.01;TNF-α:t=14.47,P＜0.01).结论大鼠去卵巢3个月时性激素水平降低,未见明显眼表损害.但泪腺中炎症因子IL-17A、IL-1β、IL-6、TNF-α的表达增加.
목적 건립거란소대서모형,관찰성격소수평개변대루선중IL-17A이급IL-1β、IL-6、TNF-α등염증인자표체적영향.방법 실험연구.건강자성SD대서20지수궤분위2조,실험조(10지)적제대서쌍측란소,대조조(10지)위미적제란소적모의수술조.술전급술후제1개월、제2개월、제3개월시2조균행루액분비시험(SIT)、각막형광염색검사진행안표평고.우제3개월시방사면역분석법분별검측2조대서혈청자이순、고동함량,HE염색관찰루선상피세포형태,면역조화염색법、Western Blot법검측2조루선중IL-17A、IL-1β、IL-6、TNF-α적표체.2조성격소질량농도비교、염증인자A치표체비교균응용독립양본t검험.2조각시간점적SIT결과비교채용중복측량자료량인소방차분석.결과 실험조대서거란소후제3개월시,체내자이순농도저우대조조(t=-35.37,P＜0.01),고동농도저우대조조(t=-12.13,P＜0.01).2조각시기SIT검사미견루액분비감소、균미견각막형광염색.HE염색견실험조루선선포위축,배렬문란,포질내매원과립명현감소.루선IL-17A표체량2조비교실험조고우대조조(면역조화법:t=7.56,P＜0.01;Western Blot법:t=20.90,P＜0.01).여각인자표체량실험조야균고우대조조(면역조화법:IL-1β:t=13.71,P＜0.01;IL-6:t=13.92,P＜0.01;TNF-α:t=6.11,P＜0.01.Western Blot법:IL-1β:t=16.93,P＜0.01;IL-6:t=12.46,P＜0.01;TNF-α:t=14.47,P＜0.01).결론 대서거란소3개월시성격소수평강저,미견명현안표손해.단루선중염증인자IL-17A、IL-1β、IL-6、TNF-α적표체증가.
Objective To study the expression of inflammatory factors IL-17A,IL-1β,IL-6 and TNF-α in the lachrymal glands of ovariectomized rats when the level of sex hormones changes.Methods This was an experimental study.Twenty healthy female SD rats were divided randomly into a control group (n=10) and an experimental group (n=10).Ovariectomy was performed on the experimenal group,while a sham operation was performed on the control group.A Schirmer Ⅰ test (SIT) and corneal fluorescence staining were performed on both groups before surgery and at 1,2,and 3 months after surgery.The results were analyzed with the repeated measurement ANOVA.Acinar cells from the lacrimal glands were examined by HE staining.At the 3rd month,the concentrations of estrogen and androgen were measured by radiation immunoassay detection and were analyzed with independent sample t tests.The expressions of IL-17A,IL-1β,IL-6 and TNF-α were analyzed by immunohistochemical and Western blot methods with independent sample t tests.Results The concentration of estrogen and androgen in the experimental group decreased compared with the control group (t=-35.37,-12.13,P＜0.01).There were no differences between the control group and the experimental group for the SIT and corneal fluorescence staining at each time point (P＞0.05).HE staining showed that the enzymes of the original particles in the lachrymal cells were significantly reduced while acinar atrophy and cell arrangement disorder were observed in the experimental group.Immunohistochemical analysis showed that the expression of inflammatory factors in the lachrymal glands was significantly higher in the experimental group compared with those in the control group (A value:IL-17A:t=7.56,P＜0.01,IL-1β:t=13.71,P＜0.01,IL-6:t=13.92,P＜0.01,TNF-α:t=6.11,P＜0.01).Western blot analysis showed that the expression levels of inflammatory factors in the lachrymal glands were significantly higher in the experimental group compared with those in the control group (A value:IL-17A:t=20.90,P＜0.01,IL-1β:t=16.93,P＜0.01,IL-6:t=12.46,P＜0.01,TNF-α:t=14.47,P＜0.01).Conclusion The levels of estrogen and androgen in the experimental group dropped at the 3rd month postoperatively but there were no obvious clinical signs found on the ocular surface.The expression of inflammatory factors,such as IL-17A,IL-1β,IL-6 and TNF-α,increased in the lachrymal glands of ovariectomized rats.