中国生物防治学报
中國生物防治學報
중국생물방치학보
CHINESE JOURNAL OF BIOLOGICAL CONTROL
2013年
1期
31-41
,共11页
胡晓磊%何玲敏%陈雪%张胜利%栾丰刚%李增智
鬍曉磊%何玲敏%陳雪%張勝利%欒豐剛%李增智
호효뢰%하령민%진설%장성리%란봉강%리증지
球孢白僵菌%ISSR%遗传多样性%种群遗传结构
毬孢白僵菌%ISSR%遺傳多樣性%種群遺傳結構
구포백강균%ISSR%유전다양성%충군유전결구
Beauveria bassiana%ISSR%genetic diversity%population genetic structure
将在中国南方9省采集分离的568株球孢白僵菌按省份划分成9个亚种群,将其中寄主可以鉴定到目的包括8目昆虫和蜘蛛目的466株球孢白僵菌按目划分成9个亚种群,用ISSR分子标记技术进行遗传多样性和种群遗传结构分析。各种多样性指数皆显示我国南方地区球孢白僵菌的遗传多样性水平较高,种群的异质性较强。其中安徽亚种群的遗传多样性和种群异质性最高,浙江亚种群最低;鳞翅目Lepidoptera分离株的遗传多样性和种群异质性最高,直翅目Orthoptera分离株最低。广东与云南菌株间以及直翅目与蜘蛛目分离株间遗传分化系数以及遗传距离最大,安徽与湖南亚种群间以及鞘翅目与膜翅目分离株间遗传分化系数以及遗传距离最小,这些结果以及基于遗传距离的亚种群聚类分析都证明我国南方球孢白僵菌的遗传谱系与寄主来源和地理来源均无关系。我国南方球孢白僵菌的变异主要是由各省不同采集地以及采集地内部不同微生境间的差异造成的,也是由各不同寄主目内各科及各属间的变异造成的。
將在中國南方9省採集分離的568株毬孢白僵菌按省份劃分成9箇亞種群,將其中寄主可以鑒定到目的包括8目昆蟲和蜘蛛目的466株毬孢白僵菌按目劃分成9箇亞種群,用ISSR分子標記技術進行遺傳多樣性和種群遺傳結構分析。各種多樣性指數皆顯示我國南方地區毬孢白僵菌的遺傳多樣性水平較高,種群的異質性較彊。其中安徽亞種群的遺傳多樣性和種群異質性最高,浙江亞種群最低;鱗翅目Lepidoptera分離株的遺傳多樣性和種群異質性最高,直翅目Orthoptera分離株最低。廣東與雲南菌株間以及直翅目與蜘蛛目分離株間遺傳分化繫數以及遺傳距離最大,安徽與湖南亞種群間以及鞘翅目與膜翅目分離株間遺傳分化繫數以及遺傳距離最小,這些結果以及基于遺傳距離的亞種群聚類分析都證明我國南方毬孢白僵菌的遺傳譜繫與寄主來源和地理來源均無關繫。我國南方毬孢白僵菌的變異主要是由各省不同採集地以及採集地內部不同微生境間的差異造成的,也是由各不同寄主目內各科及各屬間的變異造成的。
장재중국남방9성채집분리적568주구포백강균안성빈화분성9개아충군,장기중기주가이감정도목적포괄8목곤충화지주목적466주구포백강균안목화분성9개아충군,용ISSR분자표기기술진행유전다양성화충군유전결구분석。각충다양성지수개현시아국남방지구구포백강균적유전다양성수평교고,충군적이질성교강。기중안휘아충군적유전다양성화충군이질성최고,절강아충군최저;린시목Lepidoptera분리주적유전다양성화충군이질성최고,직시목Orthoptera분리주최저。엄동여운남균주간이급직시목여지주목분리주간유전분화계수이급유전거리최대,안휘여호남아충군간이급초시목여막시목분리주간유전분화계수이급유전거리최소,저사결과이급기우유전거리적아충군취류분석도증명아국남방구포백강균적유전보계여기주래원화지리래원균무관계。아국남방구포백강균적변이주요시유각성불동채집지이급채집지내부불동미생경간적차이조성적,야시유각불동기주목내각과급각속간적변이조성적。
Five hundred and sixty-eight isolates of Beauveria bassiana collected from 9 provinces in south China were divided into 9 provincial subpopulations and 466 isolates among them divided into 9 subpopulations including 8 insect orders and Araneida. All isolates were analyzed for genetic diversity and population genetic structure using an ISSR technique. All diversity parameters showed a high level of both genetic diversity and population heterogeneity of B. bassiana. Among the geographic isolates, Anhui subpopulation and Lepidoptera subpopulation had the highest genetic diversity and the highest population heterogeneity, while Zhejiang subpopulation and Orthoptera subpopulation harbored the lowest. Genetic differentiation and genetic distance were the highest between Guangdong and Yunnan subpopulations and between Orthoptera and Araneida subpopulations, and were the lowest between Anhui and Hunan subpopulations and between Coleoptera and Hymenoptera subpopulations. These results and subpopulation clustering analyses based on genetic distance of subpopulations all demonstrated that phylogenetic relationships of B. bassiana isolates in south China was associated neither with their geographic location nor with their host origin. The variation of B. bassiana in south China resulted mainly from variation between different collection sites and microhabitats inside them, and also from variation between families and genera of hosts.