CAJ | 학술논문

　　目的：探讨8-溴-7-甲氧基白杨素（BrMC）诱导人非小细胞肺癌A549细胞凋亡作用及分子机制。方法：流式细胞术(PI染色)分别检测白杨素及BrMC处理后的细胞凋亡。蛋白印迹法检测BrMC对Bim蛋白表达以及N-乙酰半胱氨酸对BrMC介导Bim表达的影响。 siRNA沉默Bim基因后流式细胞仪检测BrMC对细胞凋亡影响。结果：BrMC对A549细胞的凋亡作用较白杨素更强。经BrMC(10μmol/L)处理后，Bim蛋白水平呈时间依赖性增加。siRNA干扰后， BrMC诱导的A549细胞凋亡率明显降低。结论：BrMC通过上调Bim诱导肺癌A549细胞的凋亡。
　　목적：탐토8-추-7-갑양기백양소（BrMC）유도인비소세포폐암A549세포조망작용급분자궤제。방법：류식세포술(PI염색)분별검측백양소급BrMC처리후적세포조망。단백인적법검측BrMC대Bim단백표체이급N-을선반광안산대BrMC개도Bim표체적영향。 siRNA침묵Bim기인후류식세포의검측BrMC대세포조망영향。결과：BrMC대A549세포적조망작용교백양소경강。경BrMC(10μmol/L)처리후，Bim단백수평정시간의뢰성증가。siRNA간우후， BrMC유도적A549세포조망솔명현강저。결론：BrMC통과상조Bim유도폐암A549세포적조망。
Objective To explore the molecular mechanism of 8-bromo-7-methoxychrysin induced apoptosis in human non-small-cell A549 cells. Methods Apoptosis of A549 cells treated with Chrysin and BrMC was mea-sured by Flow cytometry using PI staining. The effect of BrMC on Bim expression in A549 cells or cells transfect-ed with Bim siRNA was analyzed by Western blot. The role of NAC in BrMC-mediated the expression of Bim was determined by Western blot. Results BrMC had more potent effect on apoptosis in A549 cells compared with Chrysin. Treatment of A549 cells with BrMC (10 μmol/L) caused a time-dependent increase in Bim protein ex-pression. Cells transfected with siRNA specific to Bim displayed a reduced proportion of apoptotic death cells in-duced by BrMC. In addition, pretreatment with NAC (10 mmol/L) inhibited BrMC-mediated Bim expression. Conclusion Our results suggest that BrMC-induced apoptosis of A549 cells was associated with upregulation of Bim protein expression.