怀化学院学报
懷化學院學報
부화학원학보
JOURNAL OF HUAIHUA TEACHERS COLLEGE
2012年
11期
41-44
,共4页
牛友芽%欧资东%赵溶娟%吴弼%喻娟%佘朝文
牛友芽%歐資東%趙溶娟%吳弼%喻娟%佘朝文
우우아%구자동%조용연%오필%유연%사조문
蛇含委陵菜%ISSR%遗传多样性
蛇含委陵菜%ISSR%遺傳多樣性
사함위릉채%ISSR%유전다양성
Potentilla kleiniana%ISSR analysis%genetic diversity
以8个蛇含委陵菜居群为材料进行ISSR分析.从20奈ISSR引物中筛选出4条稳定性强、条带清晰及多态性丰富的引物;筛选出的ISSR引物共扩增出41条带,其中39条多态性带,多态性比率为95.2l%.用Ntsys2.10e软件进行UPGMA聚类分析.结果表明:蛇含委陵菜种质资源具有较高的遗传多样性,且其遗传多样性差异与生态分布和地理来源密切相关.
以8箇蛇含委陵菜居群為材料進行ISSR分析.從20奈ISSR引物中篩選齣4條穩定性彊、條帶清晰及多態性豐富的引物;篩選齣的ISSR引物共擴增齣41條帶,其中39條多態性帶,多態性比率為95.2l%.用Ntsys2.10e軟件進行UPGMA聚類分析.結果錶明:蛇含委陵菜種質資源具有較高的遺傳多樣性,且其遺傳多樣性差異與生態分佈和地理來源密切相關.
이8개사함위릉채거군위재료진행ISSR분석.종20내ISSR인물중사선출4조은정성강、조대청석급다태성봉부적인물;사선출적ISSR인물공확증출41조대,기중39조다태성대,다태성비솔위95.2l%.용Ntsys2.10e연건진행UPGMA취류분석.결과표명:사함위릉채충질자원구유교고적유전다양성,차기유전다양성차이여생태분포화지리래원밀절상관.
8 samples of Potentilla kleiniana from different areas were analyzed by ISSR markers. The confirmed 4 primers were screened out from 20 test primers, with highly reproducible, clear and polymorphic bands. The results showed that 39 (95.21%) polymorphic bands were obtained in the total of 41 bands. UPGMA dendrogram analyzed by NTSYS demonstrated that the diversity levels of the different PotentiUa kleiniana resources were relatively high, suggested the relationship of Potentilla kleiniana from different places were related with the geographical location and ecological distribution.