广东林业科技
廣東林業科技
엄동임업과기
FORESTRY SCIENCE AND TECHNOLOGY OF GUANGDONG PROVINCE
2014年
2期
78-81
,共4页
罗万业%罗万周%魏锦秋%陈桂琼%朱昔娇
囉萬業%囉萬週%魏錦鞦%陳桂瓊%硃昔嬌
라만업%라만주%위금추%진계경%주석교
铁皮石斛%组织培养%培植技术
鐵皮石斛%組織培養%培植技術
철피석곡%조직배양%배식기술
Dendrobium officinale%tissue culture%cultivation techniques
对铁皮石斛组织培养育苗和组培苗培植技术进行研究，结果表明：在MS培养基中添加6-BA 1．0 mg/L＋NAA 0．1 mg/L＋IAA 0．5 mg/L＋50 g/L椰子汁有利于芽苗的增殖；在1/2MS 培养基中添加6-BA 0．1 mg/L＋IBA 1．0 mg/L＋NAA 0．2 mg/L＋50 g/L椰子汁有利于小苗生根和植株生长。搭建大棚移植铁皮石斛组培苗，采用高架苗床，选用松树皮和花生壳（v/v＝4∶1）为基质，移栽成活率可达95％以上。
對鐵皮石斛組織培養育苗和組培苗培植技術進行研究，結果錶明：在MS培養基中添加6-BA 1．0 mg/L＋NAA 0．1 mg/L＋IAA 0．5 mg/L＋50 g/L椰子汁有利于芽苗的增殖；在1/2MS 培養基中添加6-BA 0．1 mg/L＋IBA 1．0 mg/L＋NAA 0．2 mg/L＋50 g/L椰子汁有利于小苗生根和植株生長。搭建大棚移植鐵皮石斛組培苗，採用高架苗床，選用鬆樹皮和花生殼（v/v＝4∶1）為基質，移栽成活率可達95％以上。
대철피석곡조직배양육묘화조배묘배식기술진행연구，결과표명：재MS배양기중첨가6-BA 1．0 mg/L＋NAA 0．1 mg/L＋IAA 0．5 mg/L＋50 g/L야자즙유리우아묘적증식；재1/2MS 배양기중첨가6-BA 0．1 mg/L＋IBA 1．0 mg/L＋NAA 0．2 mg/L＋50 g/L야자즙유리우소묘생근화식주생장。탑건대붕이식철피석곡조배묘，채용고가묘상，선용송수피화화생각（v/v＝4∶1）위기질，이재성활솔가체95％이상。
The tissue culture and cultivation techniques of Dendrobium officinale were studied,the results showed that:the MS medium supplemented with 6-BA 1 .0 mg/L +NAA 0.1 mg/L +IAA 0.5 mg/L +50 g/L coconut juice was good for bud proliferation;the 1/2MS medium supplemented with 6-BA 0.1 mg/L +IBA 1 .0 mg/L +NAA 0.2 mg/L +50 g/L coconut juice was good for rooting and growth of seedlings.The transplantion survival rate could reach above 95%,when the plantlets of D.officinale were planted under the conditions of green-house,elevated seedbed and growing media with pine bark +peanut shell (v/v =4∶1 ).
