CAJ | 학술논문

采用连续二倍梯度琼脂稀释法研究龙脷叶水提物和的50%、70%、95%乙醇提取物的抑菌活性，并对抑菌作用强的提取物以石油醚、乙酸乙酯、正丁醇依次萃取，测定不同极性部位的最低抑菌浓度（MIC）。结果表明，龙脷叶50%乙醇提取物的正丁醇部位对六种试验菌株明显抑制：对金黄色葡萄球菌（Staphylococcus aureus）、金黄色葡萄球菌耐药株（Methicillin resistant Staphylococcus aureus）和大肠杆菌（Escherichia coli）的MIC 为15.60m g·mL-1；对铜绿假单胞菌（Pseudomonas aeruginosa）、伤寒沙门氏菌（Salmonella typhi.）和乙型副伤寒沙门氏菌（Bacillus paratyphosus B）的MIC 为7.81m g·mL-1。可为进一步研究龙脷叶抑菌活性成分提供依据。
채용련속이배제도경지희석법연구룡리협수제물화적50%、70%、95%을순제취물적억균활성，병대억균작용강적제취물이석유미、을산을지、정정순의차췌취，측정불동겁성부위적최저억균농도（MIC）。결과표명，룡리협50%을순제취물적정정순부위대륙충시험균주명현억제：대금황색포도구균（Staphylococcus aureus）、금황색포도구균내약주（Methicillin resistant Staphylococcus aureus）화대장간균（Escherichia coli）적MIC 위15.60m g·mL-1；대동록가단포균（Pseudomonas aeruginosa）、상한사문씨균（Salmonella typhi.）화을형부상한사문씨균（Bacillus paratyphosus B）적MIC 위7.81m g·mL-1。가위진일보연구룡리협억균활성성분제공의거。
The minimum inhibitory concentration (MIC) of system solvent extraction by petroleum, ethyl acetate and N-butanol from water and Ethanol Extract of Sauropus spatuli folius Beille against six strains were detected by using continuous double agar dilution method. The results indicated that The N-butanol liquor from Water Extract of Sauropus spatuli folius Beille had good antibacterial activities against the six strains .The MIC were 15.60m g·mL-1 on Staphylococcus aureus、Methicillin resistant Staphylococcus aureus and Escherichia coli. The MIC were 7.81 mg·mL-1 on Pseudomonas aeruginosa ,Salmonella typhi and Bacillus paratyphosus B. Sauropus spatuli folius Beille has antibacterial effects in vitro. Antibacterial active situs mainly existed in n-butanol.