CAJ | 학술논문

目的探讨地西他滨去甲基化作用治疗急性白血病患者的有效性.方法通过甲基化特异性PCR选取10例凋亡蛋白酶活化因子1(Apaf-1)基因启动子发生甲基化的患者,经地西他滨治疗后,通过甲基化特异性PCR检测患者Apaf-1基因去甲基化状态.通过反转录PCR检测10例患者经地西他滨治疗前后骨髓单个核细胞Apaf-1基因mRNA表达情况.结果甲基化特异性PCR检测示,10例患者经地西他滨治疗后,6例发生了去甲基化.治疗前10例患者因甲基化Apaf-1 mRNA均表达缺失,经地西他滨治疗后,4例重新表达,6例未表达.治疗后6例Apaf-1 mRNA表达缺失的患者中,4例Apaf-1基因未发生去甲基化,2例发生去甲基化,考虑可能与等位基因缺失或突变有关.结论地西他滨通过使Apaf-1基因启动子去甲基化恢复抑癌基因的表达,发挥其凋亡中的关键因子作用.
목적 탐토지서타빈거갑기화작용치료급성백혈병환자적유효성.방법 통과갑기화특이성PCR선취10례조망단백매활화인자1(Apaf-1)기인계동자발생갑기화적환자,경지서타빈치료후,통과갑기화특이성PCR검측환자Apaf-1기인거갑기화상태.통과반전록PCR검측10례환자경지서타빈치료전후골수단개핵세포Apaf-1기인mRNA표체정황.결과 갑기화특이성PCR검측시,10례환자경지서타빈치료후,6례발생료거갑기화.치료전10례환자인갑기화Apaf-1 mRNA균표체결실,경지서타빈치료후,4례중신표체,6례미표체.치료후6례Apaf-1 mRNA표체결실적환자중,4례Apaf-1기인미발생거갑기화,2례발생거갑기화,고필가능여등위기인결실혹돌변유관.결론 지서타빈통과사Apaf-1기인계동자거갑기화회복억암기인적표체,발휘기조망중적관건인자작용.
Objective To investigate the effectiveness of decitabine demethylation in treatment of acute leukemia.Methods Methylation specific PCR (MSP) was used to detected the methylation status of Apaf-1 gene promoter.10 cases entering the group.MSP was used to detected the 10 cases methylation status of Apaf-1 promoter between pre-and post-treatment of dicitabine.RT-PCR method used was to detect the differential expression levels of Apaf-1 mRNA in acute leukemia bone marrow mononuclear cell between preand post-treatment of decitabine.Results In post-treatment of decitabine,6 cases Apaf-1 gene promoter was demethylated.The loss expression of Apaf-1 mRNA re-expressed in 4 cases.6 cases Apaf-1 mRNA still express deletion.6 cases patients have Apaf-1 mRNA exprssion deletion,However,4 cases Apaf-1 gene was demethylated,2 cases methylated in post-treatment,maybe related to allele deletion or allelic varriants.Conclusion Post treatment of decitabine.Apaf-1 gene promotor was demethylated and repress the expression of Apaf-1 mRNA,play a key role in apoptosis maybe a new method for treatment of acute leukemia.