CAJ | 학술논문

　　建立了一种柱前衍生反相高效液相色谱检测溶液中低浓度5-氨基乙酰丙酸的方法，填补了低浓度ALA的HPLC法检测的空白。使用Agilent SB-C18型色谱柱(250 mm×4.6 mm i.d.，5μm)，以50 mM甲醇-醋酸钠缓冲溶液(pH 4.0)洗脱，流速为1 mL/min，柱温30℃，254 nm波长下检测10 min。在此条件下，5-氨基乙酰丙酸在浓度为1~1000 mg/L范围内与色谱峰面积值之间线性关系良好，回归系数均在0.9999以上。发酵液产物的加标回收率平均值为101.7%。将检测结果与分光光度法相比，符合较好。测试数据表明，此方法简单、可靠，对于低浓度的ALA测定效果良好。
　　건립료일충주전연생반상고효액상색보검측용액중저농도5-안기을선병산적방법，전보료저농도ALA적HPLC법검측적공백。사용Agilent SB-C18형색보주(250 mm×4.6 mm i.d.，5μm)，이50 mM갑순-작산납완충용액(pH 4.0)세탈，류속위1 mL/min，주온30℃，254 nm파장하검측10 min。재차조건하，5-안기을선병산재농도위1~1000 mg/L범위내여색보봉면적치지간선성관계량호，회귀계수균재0.9999이상。발효액산물적가표회수솔평균치위101.7%。장검측결과여분광광도법상비，부합교호。측시수거표명，차방법간단、가고，대우저농도적ALA측정효과량호。
A reversed phase high performance liquid chromatography with precolumn derivation was developed for the determination of low concentration 5-aminolevulinic acid (ALA), filled the blank of low concentration of ALA detected by HPLC. An Agilent SB-C18 column (250 mm ×4.6 mm i.d., 5μm) was used for separation, and the elution was performed with methanol and 50 mmol/L sodium acetate solution (pH 4.0). The flow rate was 1 mL/min, the temperature of column was 30°C, and the detection wavelength was at 254 nm with the detection time of 10 minutes. The dynamic linear range was 1-1000 mg/L, and the correlation coefficient was above 0.9999. The average recoveries of ALA in 3 samples of fermentation were 101.7%. The results agreed well with those of spectrophotometry. So the developed method is simple and reliable for the determination of low concentration ALA.