中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
12期
62-68
,共7页
SbALDH7%耐逆性%高粱
SbALDH7%耐逆性%高粱
SbALDH7%내역성%고량
SbALDH7%stress tolerance%sorghum bicolor
旨在研究高粱(Sorghum bicolor)中的醛脱氢酶基因SbALDH7的序列信息和表达分析。通过PCR和RT-PCR方法，从高粱中克隆SbALDH7基因及其cDNA全长序列。通过网络软件，聚类分析SbALDH7的序列特征，利用半定量RT-PCR，研究其在不同组织和胁迫条件下的表达谱。结果表明：SbALDH7基因含13个内含子，共编码509个氨基酸，其编码的氨基酸序列具有典型的ALDH7结构域特征。SbALDH7的表达受到PEG、Nacl、ABA和低温等胁迫环境诱导上调。组织特异性表达分析显示，在PEG渗透胁迫环境下，SbALDH7在茎、叶中的表达量显著高于根中的表达量。抗旱性有显著差异的6个高粱品种的SbALDH7蛋白序列无差异，但高粱品种的苗期抗性与SbALDH7的表达量间存在显著的正相关，表明SbALDH7的表达对高粱的抗旱性可能起着重要的作用。SbALDH7具备醛脱氢酶家族蛋白典型结构特征，在高粱抗逆过程中起重要作用，可作为提高高粱抗旱耐盐的靶基因应用于作物遗传改良。
旨在研究高粱(Sorghum bicolor)中的醛脫氫酶基因SbALDH7的序列信息和錶達分析。通過PCR和RT-PCR方法，從高粱中剋隆SbALDH7基因及其cDNA全長序列。通過網絡軟件，聚類分析SbALDH7的序列特徵，利用半定量RT-PCR，研究其在不同組織和脅迫條件下的錶達譜。結果錶明：SbALDH7基因含13箇內含子，共編碼509箇氨基痠，其編碼的氨基痠序列具有典型的ALDH7結構域特徵。SbALDH7的錶達受到PEG、Nacl、ABA和低溫等脅迫環境誘導上調。組織特異性錶達分析顯示，在PEG滲透脅迫環境下，SbALDH7在莖、葉中的錶達量顯著高于根中的錶達量。抗旱性有顯著差異的6箇高粱品種的SbALDH7蛋白序列無差異，但高粱品種的苗期抗性與SbALDH7的錶達量間存在顯著的正相關，錶明SbALDH7的錶達對高粱的抗旱性可能起著重要的作用。SbALDH7具備醛脫氫酶傢族蛋白典型結構特徵，在高粱抗逆過程中起重要作用，可作為提高高粱抗旱耐鹽的靶基因應用于作物遺傳改良。
지재연구고량(Sorghum bicolor)중적철탈경매기인SbALDH7적서렬신식화표체분석。통과PCR화RT-PCR방법，종고량중극륭SbALDH7기인급기cDNA전장서렬。통과망락연건，취류분석SbALDH7적서렬특정，이용반정량RT-PCR，연구기재불동조직화협박조건하적표체보。결과표명：SbALDH7기인함13개내함자，공편마509개안기산，기편마적안기산서렬구유전형적ALDH7결구역특정。SbALDH7적표체수도PEG、Nacl、ABA화저온등협박배경유도상조。조직특이성표체분석현시，재PEG삼투협박배경하，SbALDH7재경、협중적표체량현저고우근중적표체량。항한성유현저차이적6개고량품충적SbALDH7단백서렬무차이，단고량품충적묘기항성여SbALDH7적표체량간존재현저적정상관，표명SbALDH7적표체대고량적항한성가능기착중요적작용。SbALDH7구비철탈경매가족단백전형결구특정，재고량항역과정중기중요작용，가작위제고고량항한내염적파기인응용우작물유전개량。
The sequence features and expression patterns of SbALDH7 were studied in Sorghum bicolor. The cDNA of SbALDH7 was cloned by RT-PCR. The structure features was analyzed by bioinformatics software. The expression patterns of the gene were investigated using Semi-quantitive RT-PCR in different tissues under different stress. The result showed that SbALDH7 included 13 introns and encoded a protein sequence with 509 amino acids. The protein deduced from SbALDH7 possesses typical characteristics of members of the ALDH7 protein family. Transcript levels of the SbALDH7 genes were upregulated by PEG, NaCl, ABA and 4℃. In PEG osmotic stress, transcript levels of the SbALDH7 gene were higher in leaf and culm than in root. There was no difference in SbALDH7 protein sequences of six sorghum varieties, which had remarkably different drought tolerance. However, there was remarkable positive correlation between seedling drought resistance of varieties and transcript levels of SbALDH7. The results showed expression of SbALDH7 was important for drought tolerance of Sorghum bicolor. SbALDH7 had typical structure features of ALDH protein family, and it played an important role during the process of stress response. SbALDH7 could be used as a target gene for crop genetic improvement.
