CAJ | 학술논문

　　旨在利用生物信息学方法鉴定大豆DGAT基因，为其功能研究奠定基础。通过生物信息学的方法，在全基因组水平上对大豆DGAT基因家族进行了鉴定和分类，并分析了其基因结构、蛋白质结构域特征、系统发生及基因表达方式。结果表明：大豆中存在10个DGAT家族基因，分别属于DGAT1、DGAT2、DGAT3亚家族，分布在6条染色体上，其中DGAT1亚家族基因主要在茎端分生组织和绿色果荚中表达；DGAT3亚家族基因主要在根中表达；而DGAT2亚家族基因表达范围最广，主要在根瘤、叶、花和绿色果荚中表达。大豆DGAT家族基因在结构及表达上的差异揭示了其功能的多样性及大豆脂肪酸代谢的复杂性，将为研究大豆的脂肪酸代谢途径提供重要的信息。
　　지재이용생물신식학방법감정대두DGAT기인，위기공능연구전정기출。통과생물신식학적방법，재전기인조수평상대대두DGAT기인가족진행료감정화분류，병분석료기기인결구、단백질결구역특정、계통발생급기인표체방식。결과표명：대두중존재10개DGAT가족기인，분별속우DGAT1、DGAT2、DGAT3아가족，분포재6조염색체상，기중DGAT1아가족기인주요재경단분생조직화록색과협중표체；DGAT3아가족기인주요재근중표체；이DGAT2아가족기인표체범위최엄，주요재근류、협、화화록색과협중표체。대두DGAT가족기인재결구급표체상적차이게시료기공능적다양성급대두지방산대사적복잡성，장위연구대두적지방산대사도경제공중요적신식。
The aim was to identify the DGAT gene in soybean with bioinformatics methods, and provide the basis for studying its function. In this study, DGAT family genes of soybean were characterized in genome wide. Their gene structures and secondary structures of proteins, as well as phylogeny and expression pattern were also investigated. Phylogenetic analysis characterized 3 classes of DGAT genes in soybean, which are belonged to DGAT1, DGAT2 and DGAT3, and they distribute in 6 chromosomes of soybean genome. Whole genome expression analysis revealed that DGAT1 subfamily genes mainly expressed in SAM and green pod while DGAT3 subfamily genes mainly in root, DGAT2 family genes broadly expressed in nodule, leaf, flower and green pod. The difference of DGAT gene structures and expression patterns suggested that the function diversity and complexity of fatty acid metabolism. These results will provide important information for the fatty acid metabolism pathway in soybean.