CAJ | 학술논문

　　为了建立盐胁迫条件下小麦叶片蛋白的双向电泳研究方法，进一步获取小麦叶片响应盐胁迫的差异蛋白图谱，以河南省主导小麦品种‘周麦18’为实验材料，利用NaCl溶液模拟盐分胁迫，在建立小麦幼苗双向电泳技术和图像分析体系的基础上，对盐胁迫下的小麦幼苗进行差异蛋白组学初步分析。结果表明：72 h 1.0%NaCl溶液胁迫下，小麦幼苗蛋白表达组发生了变化；其中共有332个点得到匹配，获得61个差异表达的蛋白点，29个表达量显著上调，32个显著下调。这表明，在盐胁迫环境条件下，植物为了满足对NaCl的需求，可能开启和关闭了体内的一些相关代谢途径。
　　위료건립염협박조건하소맥협편단백적쌍향전영연구방법，진일보획취소맥협편향응염협박적차이단백도보，이하남성주도소맥품충‘주맥18’위실험재료，이용NaCl용액모의염분협박，재건립소맥유묘쌍향전영기술화도상분석체계적기출상，대염협박하적소맥유묘진행차이단백조학초보분석。결과표명：72 h 1.0%NaCl용액협박하，소맥유묘단백표체조발생료변화；기중공유332개점득도필배，획득61개차이표체적단백점，29개표체량현저상조，32개현저하조。저표명，재염협박배경조건하，식물위료만족대NaCl적수구，가능개계화관폐료체내적일사상관대사도경。
The aims were to set up an approach on two-dimensional electrophoresis for wheat leaf proteins under salt stress, and obtain the discrepancy-expressed protein map responded to high-salt content in soil. For this study, the experimental material was a main wheat cultivar‘Zhoumai-18’in Henan, NaCl solution was utilized to imitate the salt stress. Based on the establishment of two dimensional electrophoresis and image analysis, the proteomic difference of wheat seedlings under salt stress was initially analyzed. The results showed that, the whole proteins extracted from young seedlings cultured in pots under 1.0%NaCl for 72 hours were changed. 332 proteins were matched and 61 proteins showed differently expressed under salt stress, with 29 up and 32 down. This indicated plants maybe turn some relevant metabolic pathways inside them on and off under salt stress in order to satisfy its need for NaCl.