CAJ | 학술논문

　　对已在NCBI上注册的普通小麦、蓝粒小麦、天蓝偃麦草查尔酮合成酶(chalcone synthase，CHS)核酸和氨基酸序列进行分析。利用生物信息学软件研究3种作物查尔酮合成酶的酶学特性，对其组成成分、理化性质、亚细胞定位、跨膜结构、分子进化、蛋白质二级结构和结构域进行预测和推断。3种作物查尔酮合成酶基因长度均约为1.2 kb，编码394个氨基酸，三者氨基酸同源性很高，在蛋白质的其他预测中，发现三者均为不稳定蛋白，疏水性较强，二级结构以α-螺旋为主，但其他结构中有所差异，在进化树中可以看出蓝粒小麦与长穗偃麦草亲缘关系较近。通过分析发现，小麦类作物查尔酮合成酶有着与其他植物中该酶相同的特性，为进一步研究其他特殊的小麦类作物相关酶及其基因提供理论依据。
　　대이재NCBI상주책적보통소맥、람립소맥、천람언맥초사이동합성매(chalcone synthase，CHS)핵산화안기산서렬진행분석。이용생물신식학연건연구3충작물사이동합성매적매학특성，대기조성성분、이화성질、아세포정위、과막결구、분자진화、단백질이급결구화결구역진행예측화추단。3충작물사이동합성매기인장도균약위1.2 kb，편마394개안기산，삼자안기산동원성흔고，재단백질적기타예측중，발현삼자균위불은정단백，소수성교강，이급결구이α-라선위주，단기타결구중유소차이，재진화수중가이간출람립소맥여장수언맥초친연관계교근。통과분석발현，소맥류작물사이동합성매유착여기타식물중해매상동적특성，위진일보연구기타특수적소맥류작물상관매급기기인제공이론의거。
The chalcone synthase genes of common wheat, blue-grained wheat, and quack grass were analyzed. The composition of amino acid sequences, molecular structure, physical and chemical characters, subcellular localization, transmembrane helices, and molecular phylogenetic evolution, secondary and tertiary structure from NCBI were analyzed and predicted by bioinformatics software. The results demonstrated that the length of three CHS genes were all 1.2 kb, which coded 394 aa and had a high homology amino acid, and they were all unstable protein, strong hydrophobic, secondary structures of the protein were most of alpha helix, but the structure had many differences, in the other prediction protein. In the evolutionary tree could be seen the blue-grained wheat and quack grass genetic relationship is close. The result implied the properties of CHS in the Triticeae crops was almost as the same as them of other plants. This study provided the basis of research the enzymes and their genes in further step.