中国农学通报
中國農學通報
중국농학통보
CHINESE AGRICULTURAL SCIENCE BULLETIN
2013年
12期
217-220
,共4页
冷鲜五花肉%假单胞菌%Gompertz模型%平方根模型%Arrhenius模型
冷鮮五花肉%假單胞菌%Gompertz模型%平方根模型%Arrhenius模型
랭선오화육%가단포균%Gompertz모형%평방근모형%Arrhenius모형
chilled marbled meat%Pseudomonas%Gompertz model%square-root model%Arrhenius model
为了建立冷鲜五花肉中假单胞菌的生长预测模型,采用选择性培养基CFC琼脂对假单胞菌进行平板计数。利用Gompertz模型拟合0、5、10、15、20℃下冷鲜五花肉中假单胞菌的生长曲线,得到假单胞菌生长预测一级模型;生长预测二级模型以平方根模型、Arrhenius模型拟合一级模型中所得动力学参数。结果表明:一级模型中回归系数R2>0.99,准确因子(Af)、偏差因子(Bf)都接近1.0,延滞期(LPD)1.6996~0.1212,最大比生长速率(U)0.5782~3.3751。二级模型中平方根模型对延滞期和最大比生长速率回归系数R2为0.9825和0.934均略高于Arrhenius模型。可用平方根预测0~20℃范围内假单胞菌的变化情况,为冷鲜五花肉中腐败微生物的预测研究提供基础。
為瞭建立冷鮮五花肉中假單胞菌的生長預測模型,採用選擇性培養基CFC瓊脂對假單胞菌進行平闆計數。利用Gompertz模型擬閤0、5、10、15、20℃下冷鮮五花肉中假單胞菌的生長麯線,得到假單胞菌生長預測一級模型;生長預測二級模型以平方根模型、Arrhenius模型擬閤一級模型中所得動力學參數。結果錶明:一級模型中迴歸繫數R2>0.99,準確因子(Af)、偏差因子(Bf)都接近1.0,延滯期(LPD)1.6996~0.1212,最大比生長速率(U)0.5782~3.3751。二級模型中平方根模型對延滯期和最大比生長速率迴歸繫數R2為0.9825和0.934均略高于Arrhenius模型。可用平方根預測0~20℃範圍內假單胞菌的變化情況,為冷鮮五花肉中腐敗微生物的預測研究提供基礎。
위료건립랭선오화육중가단포균적생장예측모형,채용선택성배양기CFC경지대가단포균진행평판계수。이용Gompertz모형의합0、5、10、15、20℃하랭선오화육중가단포균적생장곡선,득도가단포균생장예측일급모형;생장예측이급모형이평방근모형、Arrhenius모형의합일급모형중소득동역학삼수。결과표명:일급모형중회귀계수R2>0.99,준학인자(Af)、편차인자(Bf)도접근1.0,연체기(LPD)1.6996~0.1212,최대비생장속솔(U)0.5782~3.3751。이급모형중평방근모형대연체기화최대비생장속솔회귀계수R2위0.9825화0.934균략고우Arrhenius모형。가용평방근예측0~20℃범위내가단포균적변화정황,위랭선오화육중부패미생물적예측연구제공기출。
In order to establish the predictive models, the plate count of Pseudomonas with selective medium (CFC agar) was developed. The predictive growth primary model of Pseudomonas was got through fitting the growth kinetics curve of chilled marbled meat under 0-20℃. The Square-root model and Arrhenius model were describe d the relationship between temperature and maximum specific growth rate, lag phase, and built the predictive secondary model of Pseudomonas. The results showed that, the regression coefficient (R2>0.99), accurate factor (Af) and bias factor (Bf) were close to 1.0, the lag phase (LPD) and maximum specific growth rate (U) ranged from 1.6996 to 0.1212 and from 0.5782 to 3.3751, respectively. Moreover, the regression coefficient (R2) of the maximum specific growth rate and lag phase in Square-root model was higher than Arrhenius model, the growth kinetics model provided a useful and accurate method of predicting the growth changes of Pseudomonas from 0℃to 20℃.