安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
1期
63-66,67
,共5页
岩白菜素%高尿酸血症%氧嗪酸钾盐%小鼠
巖白菜素%高尿痠血癥%氧嗪痠鉀鹽%小鼠
암백채소%고뇨산혈증%양진산갑염%소서
bergenin%hyperuricemia%potassium oxonate%mice
目的在氧嗪酸钾盐诱导小鼠产生高尿酸血症模型的基础上，探讨岩白菜素的抗高尿酸活性。方法将60只昆明种雄性小鼠随机均分为6组：正常对照组、高尿酸血症模型组、岩白菜素(20、40、60 mg/kg)组、别嘌呤醇(5 mg/kg)组。每天灌胃氧嗪酸钾盐(250 mg/kg)1 h后再灌胃给药，连续1周。分别测定血清中尿酸、肌酐和尿素氮水平，尿液中尿酸和肌酐水平，以及肾脏尿酸转运子1( mURAT1)和葡萄糖转运子9(mGLUT9)的mRNA和蛋白表达水平。结果与高尿酸血症模型组比较，岩白菜素显著降低高尿酸血症小鼠血清中尿酸、肌酐和尿素氮水平，提高24 h尿液尿酸和肌酐排泄量以及尿酸排泄分数，显著下调高尿酸血症小鼠肾脏mURAT1和 mGLUT9的mRNA和蛋白表达水平。结论岩白菜素通过促进高尿酸血症小鼠肾脏尿酸排泄作用及逆转尿酸转运体的过度表达，从而体现抗高尿酸活性。
目的在氧嗪痠鉀鹽誘導小鼠產生高尿痠血癥模型的基礎上，探討巖白菜素的抗高尿痠活性。方法將60隻昆明種雄性小鼠隨機均分為6組：正常對照組、高尿痠血癥模型組、巖白菜素(20、40、60 mg/kg)組、彆嘌呤醇(5 mg/kg)組。每天灌胃氧嗪痠鉀鹽(250 mg/kg)1 h後再灌胃給藥，連續1週。分彆測定血清中尿痠、肌酐和尿素氮水平，尿液中尿痠和肌酐水平，以及腎髒尿痠轉運子1( mURAT1)和葡萄糖轉運子9(mGLUT9)的mRNA和蛋白錶達水平。結果與高尿痠血癥模型組比較，巖白菜素顯著降低高尿痠血癥小鼠血清中尿痠、肌酐和尿素氮水平，提高24 h尿液尿痠和肌酐排洩量以及尿痠排洩分數，顯著下調高尿痠血癥小鼠腎髒mURAT1和 mGLUT9的mRNA和蛋白錶達水平。結論巖白菜素通過促進高尿痠血癥小鼠腎髒尿痠排洩作用及逆轉尿痠轉運體的過度錶達，從而體現抗高尿痠活性。
목적재양진산갑염유도소서산생고뇨산혈증모형적기출상，탐토암백채소적항고뇨산활성。방법장60지곤명충웅성소서수궤균분위6조：정상대조조、고뇨산혈증모형조、암백채소(20、40、60 mg/kg)조、별표령순(5 mg/kg)조。매천관위양진산갑염(250 mg/kg)1 h후재관위급약，련속1주。분별측정혈청중뇨산、기항화뇨소담수평，뇨액중뇨산화기항수평，이급신장뇨산전운자1( mURAT1)화포도당전운자9(mGLUT9)적mRNA화단백표체수평。결과여고뇨산혈증모형조비교，암백채소현저강저고뇨산혈증소서혈청중뇨산、기항화뇨소담수평，제고24 h뇨액뇨산화기항배설량이급뇨산배설분수，현저하조고뇨산혈증소서신장mURAT1화 mGLUT9적mRNA화단백표체수평。결론암백채소통과촉진고뇨산혈증소서신장뇨산배설작용급역전뇨산전운체적과도표체，종이체현항고뇨산활성。
Objective To explore the anti-hyperuricemia activity of bergenin in the model of hyperuricemic mice induced by potassium oxonate. Methods 60 Kunming male mice were divided randomly into six groups, which were normal control group;hyperuricemic model group;and hyperuricemic groups with 20 , 40 , 60 mg/kg berge-nin, and 5 mg/kg allopurinol. Mice were orally administered once daily with 250 mg/kg potassium oxonate for 7 continuous days to create the model, and then three doses of bergenin and allopurinol were orally initiated on the day 1 h after potassium oxonate was given, separately. Serum uric acid, creatinine and urea nitrogon levels, as well as urinary uric acid and creatinine levels were measured. mRNA and protein expression levels of mouse kidney u-rate transporter 1(URAT1), and glucose transporter 9(GLUT9) were also determined. Results Compared with hyperuricemic model group, bergenin significantly reduced serum uric acid, creatinine and urea nitrogon levels, in-creased 24 h uric acid and creatinine excretion, and fractional excretion of uric acid in hyperuricemic mice;mRNA and protein levels of mURAT1 and mGLUT9 were also markedly down-regulated. Conclusion Anti-hyperuricemia effect of bergenin is attributed to the enhancement of uric acid excretion and reversion of mouse urate transporters o-ver-expression.
