华南农业大学学报
華南農業大學學報
화남농업대학학보
JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY
2014年
3期
24-29
,共6页
李磊%张英楠%杨树宝%冯小刚%刘莹%董红艳%马金萍%栾维民
李磊%張英楠%楊樹寶%馮小剛%劉瑩%董紅豔%馬金萍%欒維民
리뢰%장영남%양수보%풍소강%류형%동홍염%마금평%란유민
嗉囊%发育%定位分布%肠相关淋巴组织
嗉囊%髮育%定位分佈%腸相關淋巴組織
소낭%발육%정위분포%장상관림파조직
crop%development%location and distribution%gut-associated lymphoid tissue ( GALT)
【目的】研究鸡出壳后不同日龄嗉囊中T淋巴细胞及其亚群和B淋巴细胞的定位分布及数量变化规律等一系列的发育过程.【方法】采用单克隆抗体,通过免疫组织化学方法,观察并统计各取材日龄相应细胞的分布和数量.【结果和结论】1日龄时, CD3+、CD4+和CD8+细胞即已出现, Bu-1 b+细胞于出壳后4日龄时出现.定位分布上, CD3+细胞主要分布于黏膜上皮基底层及固有层中,7日龄首次出现以CD3+细胞为中心的淋巴聚集物;CD4+细胞主要分布于固有层中;CD8+细胞最初主要分布在黏膜固有层中,随日龄增长逐渐向上皮方向突入,最终形成含有CD8+细胞浸润的淋巴上皮;Bu-1b+细胞主要分布于黏膜固有层中,特别是在小血管周围.于35日龄观察到以Bu-1b+细胞为中心的生发中心.在数量变化上,CD3+、CD4+、CD8+细胞数量整体呈逐渐增加趋势,部分阳性细胞数量在14和21日龄时稍有下降,35~56日龄时阳性细胞数量缓慢增加,基本达到成熟水平.Bu-1b+细胞数量随着日龄的增加持续增多,且后期的增长速率大于T细胞的增长速率.结果表明,嗉囊作为鸡消化系统的一部分,具有由淋巴细胞和淋巴聚集物构成的免疫防线,为肠相关淋巴组织中的一个重要组成部分.
【目的】研究鷄齣殼後不同日齡嗉囊中T淋巴細胞及其亞群和B淋巴細胞的定位分佈及數量變化規律等一繫列的髮育過程.【方法】採用單剋隆抗體,通過免疫組織化學方法,觀察併統計各取材日齡相應細胞的分佈和數量.【結果和結論】1日齡時, CD3+、CD4+和CD8+細胞即已齣現, Bu-1 b+細胞于齣殼後4日齡時齣現.定位分佈上, CD3+細胞主要分佈于黏膜上皮基底層及固有層中,7日齡首次齣現以CD3+細胞為中心的淋巴聚集物;CD4+細胞主要分佈于固有層中;CD8+細胞最初主要分佈在黏膜固有層中,隨日齡增長逐漸嚮上皮方嚮突入,最終形成含有CD8+細胞浸潤的淋巴上皮;Bu-1b+細胞主要分佈于黏膜固有層中,特彆是在小血管週圍.于35日齡觀察到以Bu-1b+細胞為中心的生髮中心.在數量變化上,CD3+、CD4+、CD8+細胞數量整體呈逐漸增加趨勢,部分暘性細胞數量在14和21日齡時稍有下降,35~56日齡時暘性細胞數量緩慢增加,基本達到成熟水平.Bu-1b+細胞數量隨著日齡的增加持續增多,且後期的增長速率大于T細胞的增長速率.結果錶明,嗉囊作為鷄消化繫統的一部分,具有由淋巴細胞和淋巴聚集物構成的免疫防線,為腸相關淋巴組織中的一箇重要組成部分.
【목적】연구계출각후불동일령소낭중T림파세포급기아군화B림파세포적정위분포급수량변화규률등일계렬적발육과정.【방법】채용단극륭항체,통과면역조직화학방법,관찰병통계각취재일령상응세포적분포화수량.【결과화결론】1일령시, CD3+、CD4+화CD8+세포즉이출현, Bu-1 b+세포우출각후4일령시출현.정위분포상, CD3+세포주요분포우점막상피기저층급고유층중,7일령수차출현이CD3+세포위중심적림파취집물;CD4+세포주요분포우고유층중;CD8+세포최초주요분포재점막고유층중,수일령증장축점향상피방향돌입,최종형성함유CD8+세포침윤적림파상피;Bu-1b+세포주요분포우점막고유층중,특별시재소혈관주위.우35일령관찰도이Bu-1b+세포위중심적생발중심.재수량변화상,CD3+、CD4+、CD8+세포수량정체정축점증가추세,부분양성세포수량재14화21일령시초유하강,35~56일령시양성세포수량완만증가,기본체도성숙수평.Bu-1b+세포수량수착일령적증가지속증다,차후기적증장속솔대우T세포적증장속솔.결과표명,소낭작위계소화계통적일부분,구유유림파세포화림파취집물구성적면역방선,위장상관림파조직중적일개중요조성부분.
[Objective]To study the appearance , distribution and quantity variation of immunocytes-T lymphocytes and its subpopulations and B lymphocytes in different periods , analyzing the developmental process of immunocytes in the chicken crop .[Method]The mono-antibodies and immunohistochemistry was used to observe the distribution and count the number of the relevant cell in every age .[Result and conclusion]The results showed that CD3 +, CD4 +and CD8 +cells appeared in the first day after post-hatching .Bu-1 b+cells were observed in the 4 rh day .CD3 +cells mainly distributed in the epithelium and lamina propria , and the lymphoid aggregates covered by CD 3 +cells were first observed in the 7 th day af-ter post-hatching.CD4 +cells were located in the lamina propria .At first, CD8 +cells mainly distributed in the lamina propria of the mucosa .With the age increasing , CD8 +cells slightly migrated and infiltrated to the epithelium of the mucosa , which were transformed to the lymphoepithelium .Bu-1 b+cells mainly distributed in the lamina propria , especially around the vessels .The germinal center which was covered by the Bu-1a +cells was first observed in the 35th day after post-hatching.The amount of CD3 +, CD4 +, and CD8 +cells kept increasing with the age; the amount of parts of positive cells decreased in the 14th and 21th day;it slightly increased after this date and reached mature level around the 56th day.Bu-1b+cells continuously kept increasing with age , its rising rate was higher than T lymphocytes during the later period.The research showed that as a part of the digest system in the chicken , the crop posses ses the mucosal immunity which consists of the lymphoid aggregates and the lymphocytes .The crop is an impor-tant part of gut-associated lymphoid tissue ( GALT) .