CAJ | 학술논문

采用单纯胰蛋白酶消化法分离,差速贴壁法纯化新生大鼠心肌细胞。光学显微镜观察心肌细胞基本形态特征,台盼蓝染色检测成活率,肌钙蛋白玉、α-横纹肌肌动蛋白抗体鉴定心肌细胞。结果显示培养24 h后心肌细胞大部分已贴壁,并出现自发搏动现象,48 h后逐渐伸出伪足相互接触交织成网,72 h后逐渐形成细胞簇并出现同步搏动；台盼蓝染色法检测细胞成活率达90%以上；肌钙蛋白玉及α-横纹肌肌动蛋白免疫荧光染色法鉴定心肌细胞纯度达98%以上。
채용단순이단백매소화법분리,차속첩벽법순화신생대서심기세포。광학현미경관찰심기세포기본형태특정,태반람염색검측성활솔,기개단백옥、α-횡문기기동단백항체감정심기세포。결과현시배양24 h후심기세포대부분이첩벽,병출현자발박동현상,48 h후축점신출위족상호접촉교직성망,72 h후축점형성세포족병출현동보박동；태반람염색법검측세포성활솔체90%이상；기개단백옥급α-횡문기기동단백면역형광염색법감정심기세포순도체98%이상。
Neonatal rat myocytes were digested with typsin and purified by technique of differential anchoring. The cardiomyocytes cultured were observed under light microscope, stained with trypan blue to count the living cells and identified with cTnI andα-Sarcomeric actin. The results showed most of cardiomyocytes adherented to culture plates after 24 hours of culture and a part of cells were jumping synchronously,48 hours later cells gradually extended pseudopodia,contacted with each other and interwovened into a network. After 72 hours of culture, the cells gradu-ally formed cell clusters and were beating synchronously. Trypan blue staining showed the survival rate of cardio-myocytes was more than 90%. cTnI andα-Sarcomeric actin immunofluorescence staining showed that the purity of cardiomyocytes cultured was more than 98%.