浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2014年
2期
115-117
,共3页
饶高峰%沈屹%吴金友%陈恩富%曾爱平
饒高峰%瀋屹%吳金友%陳恩富%曾愛平
요고봉%침흘%오금우%진은부%증애평
老年人2型糖尿病%肿瘤坏死因子%单核苷酸多态性
老年人2型糖尿病%腫瘤壞死因子%單覈苷痠多態性
노년인2형당뇨병%종류배사인자%단핵감산다태성
Type 2 diabetes mellitus in elderly patients%Tumor necrosis factor- α%SNP
目的：研究TNF-α基因多态性在浙江台州地区2型糖尿病患者中的分布与相关性。方法利用DNA测序法对100例老年2型糖尿病患者（观察组）及50例健康体检者（对照组）的TNF-α基因-308位点和-238位点进行基因检测与分析。结果观察组FPG、TNF-α、TG、TC、胰岛素抵抗指数（IRI）分别为（10.68±2.75）mmol/L、（2.01±0.25）ug/L、（2.43±1.15）mmol/L、（5.49±1.63）mmol/L及4.65±0.97，均高于对照组，差异均有统计学意义（均P＜0.05），HDL为（1.23±0.28）mmol/L，低于对照组，差异有统计学意义（P＜0.05）。而BMI、LDL[（25.34±2.41）kg/m2、（3.26±1.3）mmol/L]与对照组比较，差异无统计学意义（P＞0.05）。TNF-α基因-308位点在观察组和对照组GA+AA基因频率分别为31%和10%，A等位基因频率分别为16%和5%，差异有统计学意义（P＜0.05）。而-238位点未见突变，两组间比较无明显差异。结论 TNF-α基因G-308A单核苷酸多态性与2型糖尿病存在相关性。
目的：研究TNF-α基因多態性在浙江檯州地區2型糖尿病患者中的分佈與相關性。方法利用DNA測序法對100例老年2型糖尿病患者（觀察組）及50例健康體檢者（對照組）的TNF-α基因-308位點和-238位點進行基因檢測與分析。結果觀察組FPG、TNF-α、TG、TC、胰島素牴抗指數（IRI）分彆為（10.68±2.75）mmol/L、（2.01±0.25）ug/L、（2.43±1.15）mmol/L、（5.49±1.63）mmol/L及4.65±0.97，均高于對照組，差異均有統計學意義（均P＜0.05），HDL為（1.23±0.28）mmol/L，低于對照組，差異有統計學意義（P＜0.05）。而BMI、LDL[（25.34±2.41）kg/m2、（3.26±1.3）mmol/L]與對照組比較，差異無統計學意義（P＞0.05）。TNF-α基因-308位點在觀察組和對照組GA+AA基因頻率分彆為31%和10%，A等位基因頻率分彆為16%和5%，差異有統計學意義（P＜0.05）。而-238位點未見突變，兩組間比較無明顯差異。結論 TNF-α基因G-308A單覈苷痠多態性與2型糖尿病存在相關性。
목적：연구TNF-α기인다태성재절강태주지구2형당뇨병환자중적분포여상관성。방법이용DNA측서법대100례노년2형당뇨병환자（관찰조）급50례건강체검자（대조조）적TNF-α기인-308위점화-238위점진행기인검측여분석。결과관찰조FPG、TNF-α、TG、TC、이도소저항지수（IRI）분별위（10.68±2.75）mmol/L、（2.01±0.25）ug/L、（2.43±1.15）mmol/L、（5.49±1.63）mmol/L급4.65±0.97，균고우대조조，차이균유통계학의의（균P＜0.05），HDL위（1.23±0.28）mmol/L，저우대조조，차이유통계학의의（P＜0.05）。이BMI、LDL[（25.34±2.41）kg/m2、（3.26±1.3）mmol/L]여대조조비교，차이무통계학의의（P＞0.05）。TNF-α기인-308위점재관찰조화대조조GA+AA기인빈솔분별위31%화10%，A등위기인빈솔분별위16%화5%，차이유통계학의의（P＜0.05）。이-238위점미견돌변，량조간비교무명현차이。결론 TNF-α기인G-308A단핵감산다태성여2형당뇨병존재상관성。
Objective To investigate the polymorphism of TNF- αgene in patients with type 2 diabetes mellitus (T2DM) in Taizhou region of Zhejiang province. Methods Blood specimens were col ected from 100 elderly patients with type 2 diabetes mel itus and 50 normal elderly subjects from Taizhou region of Zhejiang Province. Genomic DNA was extracted from blood sam-ples. The primers of PCR for the SNPs of G308A and G238A were optimized and the polymorphism of amplicons was analyzed by DNA sequencing. Results FPG, TNF- α, TG, TC and insulin resistance index in T2DM group were 10.68±2.75mmol/L, 2.01± 0.25μg/L, 2.43±1.15mmol/L, 5.49±1.63mmol/L and 4.65±0.97, respectively, which were al higher than those in control group (P<0.05). The HDL- C(1.23±0.28mmol/L) was lower than that in controls(P<0.05), while there was no difference in BMI(25.34± 2.41kg/m2) and LDL(3.26±1.3mmol/L) between T2DM patients and controls (P>0.05).The frequency of GA+AA genotype in TNF- α- 308 gene of T2DM group and control group was 31%and 10%,and A al ele 16%and 5%, respectively (P<0.05);while there was no difference in genotype and A al ele frequency in TNF- a- 238 gene between two groups. Conclusion The 308 G- A polymorphism of TNF- αpromoter gene may be associated with type 2 diabetes mel itus in elderly patients from Taizhou region of Zhejiang province.
