CAJ | 학술논문

目的：通过观察大鼠脑组织中水通道蛋白AQP-4的表达变化，探讨水通道蛋白4表达的变化与高湿环境的关系。方法32只健康雄性的SD大鼠，随机分为30℃20d组、30℃30d组和30℃40d组(采用人工气候箱制作高湿模型)和常温常湿对照组，每组8只。采用实时定量PCR测定大鼠脑组织AQP-4 mRNA表达， Western印迹法观察大鼠脑组织AQP-4蛋白表达。结果高湿环境下30℃20d组、30℃30d组和30℃40d组脑组织AQP-4 mRNA、蛋白水平与对照组相比下降，而只有30℃40d组脑组织AQP-4 mRNA、蛋白水平与对照组相比下降差异有统计学意义( P<0.05)。结论脑组织AQP-4表达的变化与高湿环境有关。
목적：통과관찰대서뇌조직중수통도단백AQP-4적표체변화，탐토수통도단백4표체적변화여고습배경적관계。방법32지건강웅성적SD대서，수궤분위30℃20d조、30℃30d조화30℃40d조(채용인공기후상제작고습모형)화상온상습대조조，매조8지。채용실시정량PCR측정대서뇌조직AQP-4 mRNA표체， Western인적법관찰대서뇌조직AQP-4단백표체。결과고습배경하30℃20d조、30℃30d조화30℃40d조뇌조직AQP-4 mRNA、단백수평여대조조상비하강，이지유30℃40d조뇌조직AQP-4 mRNA、단백수평여대조조상비하강차이유통계학의의( P<0.05)。결론뇌조직AQP-4표체적변화여고습배경유관。
Objective To study the changes of expression of AQP-4 in the brain of rat in high humid environment. Meth-ods 32 healthy male SD rat were evenly randomized into 4groups:30℃ 20 day group, 30℃ 30 day group, 30℃ 40 day group and control group. Rats in high humid environment groups were exposed in an artificial environment chamber with ( RH) >90%. Real-time PCR and Western blotting assay were used to observe the expression changes of AQP-4 mRNA and protein in the brain of all the animals, respectively. Results The expression of AQP-4 mRNA and protein in the brain of 30℃ 40 day group signifi-cantly decreased compared to that of the control group. Conclusion The high humid environment has an effect on the changes of expression of AQP-4 in the brain.