CAJ | 학술논문

目的：研究类姜黄素（curcuminoids, COM）及其单体成分姜黄素(curcumin, Cur)、去甲氧基姜黄素(de-methoxycurcumin, DMC)、双去甲氧基姜黄素(bisdemethoxy-curcumin, BDMC)）对β-内分泌酶活性的影响，以此探讨其抑制β-淀粉样蛋白（Aβ42）生成的机制。方法在swAPP-HEK293细胞中，依次分为空白对照组、COM组、Cur组、DMC组和BDMC组（药物浓度为5μM），药物培养作用24 h后，采用MTT法检测细胞的增殖状况，运用BioVision公司的β-内分泌酶活性荧光检测试剂盒测定β-内分泌酶的相对活性。结果5μM浓度的类姜黄素及单体成分对细胞活性无明显影响；类姜黄素及其三种单体成分均可明显抑制β-内分泌酶的活性，抑制强弱依次为Cur>DMC>COM>BDMC。结论类姜黄素及其单体成分可通过抑制β-内分泌酶的活性而实现其抑制Aβ的作用。
목적：연구류강황소（curcuminoids, COM）급기단체성분강황소(curcumin, Cur)、거갑양기강황소(de-methoxycurcumin, DMC)、쌍거갑양기강황소(bisdemethoxy-curcumin, BDMC)）대β-내분비매활성적영향，이차탐토기억제β-정분양단백（Aβ42）생성적궤제。방법재swAPP-HEK293세포중，의차분위공백대조조、COM조、Cur조、DMC조화BDMC조（약물농도위5μM），약물배양작용24 h후，채용MTT법검측세포적증식상황，운용BioVision공사적β-내분비매활성형광검측시제합측정β-내분비매적상대활성。결과5μM농도적류강황소급단체성분대세포활성무명현영향；류강황소급기삼충단체성분균가명현억제β-내분비매적활성，억제강약의차위Cur>DMC>COM>BDMC。결론류강황소급기단체성분가통과억제β-내분비매적활성이실현기억제Aβ적작용。
Objective The anti-amyloidogenic effects of Curcumin had been clearly certified, Whether the anti-amyliodogenic effects were mediated through the modulation of BACE1 activity was addressed. Methods SwAPP-HEK293 cells were incubated for 24h without or with 5μM curcumin mix, Cur, DMC and BDMC respectively, then the cells were collected for the cell viability evaluation by using MTT analysis and for the BACE1 activity evaluation by theβ-Secretase Activity Fluorometric Assay Kit. Results The cell viability of swAPPHEK293 cells was unchanged after cur-cuminoids incubation; All of Curcumin mix, Cur, DMC and BDMC could decreased the BACE1 activity, Cur was the most active in suppressing BACE1 activity, and the inhibition strength of others in order was DMC>COM>BDMC. Con-clusion This paper indicate that the anti-amyloidogenic effects of curcumin mix,Cur ,DMC,BDMC may be through the in-hibition of BACE1 activity.