生态环境学报
生態環境學報
생태배경학보
ECOLOGY AND ENVIRONMENT
2014年
1期
122-128
,共7页
曾丽璇%张悦君%康园%张秋云
曾麗璇%張悅君%康園%張鞦雲
증려선%장열군%강완%장추운
双酚A%壬基酚%河蚬%耗氧率%排氨率%SOD%CAT
雙酚A%壬基酚%河蜆%耗氧率%排氨率%SOD%CAT
쌍분A%임기분%하현%모양솔%배안솔%SOD%CAT
bisphenol a%nonylphenol%Asian clam (Corbicula fluminea)%oxygen consumption rate (OCR)%ammonia excretory rate (AER)%superoxide dismutase (SOD)%catalase (CAT)
为了研究双酚A(Bisphenol A,BPA)和壬基酚(Nonylphenol,NP)对河蚬(Corbicula fluminea)呼吸代谢能力和抗氧化酶活性的影响,探讨BPA和NP对河蚬的毒性作用。以河蚬为受试生物,采用半静态染毒法,研究了BPA和NP对河蚬的单一毒性等级、联合毒性作用类型和对河蚬耗氧率、排氨率以及抗氧化酶系统中SOD、CAT活性的影响。结果显示:① BPA、NP对河蚬的半致死质量浓度(96 h-LC50)分别为6.34和1.09 mg·L-1,毒性顺序为NP>BPA,二者均为高毒物质;② BPA-NP对河蚬毒性作用类型为协同作用;③亚急性毒性指标耗氧率、排氨率以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)对BPA、NP及二者混合物均比较敏感,在本试验的质量浓度范围内(BPA:0.63、0.79、1.59、3.17 mg·L-1;NP:0.109、0.136、0.271、0.542 mg·L-1;BPA+NP:(0.0782+0.029)、(0.0978+0.0363)、(0.196+0.0725)、(0.391+0.145) mg·L-1),河蚬耗氧率、排氨率以及两种酶活性变化均呈现先下降后上升再下降的趋势,其中在BPA、NP和二者混合物的质量浓度较低时,河蚬的耗氧率和排氨率、SOD和CAT活性只有微小降低,随着质量浓度的升高,河蚬耗氧率和排氨率、两个酶活性相对均有所升高,而在质量浓度极高时其耗氧率和排氨率以及两个酶活性才又受到显著性或极显著性的抑制。实验结果显示河蚬耗氧率、排氨率和SOD、CAT活性对水体中酚类内分泌干扰物反应敏感,具有较好的一致性和规律性,耗氧率、排氨率和SOD、CAT活性与其他敏感性指标一起可以作为酚类内分泌干扰物污染的一项早期监测指标。
為瞭研究雙酚A(Bisphenol A,BPA)和壬基酚(Nonylphenol,NP)對河蜆(Corbicula fluminea)呼吸代謝能力和抗氧化酶活性的影響,探討BPA和NP對河蜆的毒性作用。以河蜆為受試生物,採用半靜態染毒法,研究瞭BPA和NP對河蜆的單一毒性等級、聯閤毒性作用類型和對河蜆耗氧率、排氨率以及抗氧化酶繫統中SOD、CAT活性的影響。結果顯示:① BPA、NP對河蜆的半緻死質量濃度(96 h-LC50)分彆為6.34和1.09 mg·L-1,毒性順序為NP>BPA,二者均為高毒物質;② BPA-NP對河蜆毒性作用類型為協同作用;③亞急性毒性指標耗氧率、排氨率以及超氧化物歧化酶(SOD)、過氧化氫酶(CAT)對BPA、NP及二者混閤物均比較敏感,在本試驗的質量濃度範圍內(BPA:0.63、0.79、1.59、3.17 mg·L-1;NP:0.109、0.136、0.271、0.542 mg·L-1;BPA+NP:(0.0782+0.029)、(0.0978+0.0363)、(0.196+0.0725)、(0.391+0.145) mg·L-1),河蜆耗氧率、排氨率以及兩種酶活性變化均呈現先下降後上升再下降的趨勢,其中在BPA、NP和二者混閤物的質量濃度較低時,河蜆的耗氧率和排氨率、SOD和CAT活性隻有微小降低,隨著質量濃度的升高,河蜆耗氧率和排氨率、兩箇酶活性相對均有所升高,而在質量濃度極高時其耗氧率和排氨率以及兩箇酶活性纔又受到顯著性或極顯著性的抑製。實驗結果顯示河蜆耗氧率、排氨率和SOD、CAT活性對水體中酚類內分泌榦擾物反應敏感,具有較好的一緻性和規律性,耗氧率、排氨率和SOD、CAT活性與其他敏感性指標一起可以作為酚類內分泌榦擾物汙染的一項早期鑑測指標。
위료연구쌍분A(Bisphenol A,BPA)화임기분(Nonylphenol,NP)대하현(Corbicula fluminea)호흡대사능력화항양화매활성적영향,탐토BPA화NP대하현적독성작용。이하현위수시생물,채용반정태염독법,연구료BPA화NP대하현적단일독성등급、연합독성작용류형화대하현모양솔、배안솔이급항양화매계통중SOD、CAT활성적영향。결과현시:① BPA、NP대하현적반치사질량농도(96 h-LC50)분별위6.34화1.09 mg·L-1,독성순서위NP>BPA,이자균위고독물질;② BPA-NP대하현독성작용류형위협동작용;③아급성독성지표모양솔、배안솔이급초양화물기화매(SOD)、과양화경매(CAT)대BPA、NP급이자혼합물균비교민감,재본시험적질량농도범위내(BPA:0.63、0.79、1.59、3.17 mg·L-1;NP:0.109、0.136、0.271、0.542 mg·L-1;BPA+NP:(0.0782+0.029)、(0.0978+0.0363)、(0.196+0.0725)、(0.391+0.145) mg·L-1),하현모양솔、배안솔이급량충매활성변화균정현선하강후상승재하강적추세,기중재BPA、NP화이자혼합물적질량농도교저시,하현적모양솔화배안솔、SOD화CAT활성지유미소강저,수착질량농도적승고,하현모양솔화배안솔、량개매활성상대균유소승고,이재질량농도겁고시기모양솔화배안솔이급량개매활성재우수도현저성혹겁현저성적억제。실험결과현시하현모양솔、배안솔화SOD、CAT활성대수체중분류내분비간우물반응민감,구유교호적일치성화규률성,모양솔、배안솔화SOD、CAT활성여기타민감성지표일기가이작위분류내분비간우물오염적일항조기감측지표。
In the present study, the effect of BPA and NP on respiratory metabolism and antioxidant enzymes in Asian clam (Corbicula fluminea) including oxygen consumption rate (OCR), the ammonia excretory rate (AER), and the activity of antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) were studied by semi-static method test.The results showed that: ① the 96 h-LC50 of BPA and NP were 6.34 and 1.09 mg·L-1, respectively, and they were both categorized as high toxic substances; ② BPA and NP had a synergistic effect on chronic and acute toxicity; ③ In the range of the experimental concentrations(BPA:0.63, 0.79 , 1.59, 3.17 mg·L-1;NP:0.109, 0.136, 0.271, 0.542 mg·L-1;BPA+NP:(0.078 2+0.029), (0.097 8+0.036 3), (0.196+0.072 5), (0.391+0.145) mg·L-1), the response of OCR, AER, SOD and CAT to BPA and NP showed a typical pattern. There was only a minor decrease for the OCR, AER, SOD and CAT at low concentrations of BPA, NP and the mixture, and these activities were up-regulated as the concentrations of pollutants were increased, but they were significantly inhibited when the concentration of pollutants were increased up to 1/2 LC50. The present studies indicated that the OCR, AER, SOD and CAT in clams could be considered as an indicator for pEDCs pollution in early stage.