CAJ | 학술논문

采用氯仿-甲醇混合液提取法及气相色谱-质谱联用法,首次对造礁石珊瑚(风信子鹿角珊瑚Acropora hyacinthus)繁殖前后脂质与脂肪酸的变化进行了研究。研究结果表明：1)繁殖过程对风信子鹿角珊瑚脂质含量、组织含量有较大影响。繁殖过后风信子鹿角珊瑚脂质含量减少约43.4%,组织含量减少约19.6%,而共生藻密度在繁殖过程中并无显著性变化；2)风信子鹿角珊瑚在繁殖前脂质含量大量累积,以顺利完成繁殖过程；3)首次表明二十碳五烯酸(C20：5n-3, eicosapntemacnioc acid, EPA)、二十二碳六烯酸(C22：6n-3, docosahexaenoic acid, DHA)是造礁石珊瑚繁殖过程中重要的脂肪酸,其含量在繁殖过程中具有显著变化。EPA含量繁殖前从15.7μg·gdw-1增加到22.16μg·gdw-1,增加41.1%,而繁殖后降为15.06μg·gdw-1,减少32.0%；DHA含量繁殖前从18.17μg·gdw-1增加到24.81μg·gdw-1,增加36.5%,而繁殖后降为16.31μg·gdw-1,减少34.3%。此外,多聚不饱和脂肪酸(poly-unsaturated fatty acids, PUFAs)含量在繁殖后也显著性减少；4)造礁石珊瑚繁殖时可能会适当增加摄食,以满足繁殖过程对脂肪酸和能量的需求。
채용록방-갑순혼합액제취법급기상색보-질보련용법,수차대조초석산호(풍신자록각산호Acropora hyacinthus)번식전후지질여지방산적변화진행료연구。연구결과표명：1)번식과정대풍신자록각산호지질함량、조직함량유교대영향。번식과후풍신자록각산호지질함량감소약43.4%,조직함량감소약19.6%,이공생조밀도재번식과정중병무현저성변화；2)풍신자록각산호재번식전지질함량대량루적,이순리완성번식과정；3)수차표명이십탄오희산(C20：5n-3, eicosapntemacnioc acid, EPA)、이십이탄륙희산(C22：6n-3, docosahexaenoic acid, DHA)시조초석산호번식과정중중요적지방산,기함량재번식과정중구유현저변화。EPA함량번식전종15.7μg·gdw-1증가도22.16μg·gdw-1,증가41.1%,이번식후강위15.06μg·gdw-1,감소32.0%；DHA함량번식전종18.17μg·gdw-1증가도24.81μg·gdw-1,증가36.5%,이번식후강위16.31μg·gdw-1,감소34.3%。차외,다취불포화지방산(poly-unsaturated fatty acids, PUFAs)함량재번식후야현저성감소；4)조초석산호번식시가능회괄당증가섭식,이만족번식과정대지방산화능량적수구。
Lipid and fatty acid contents were firstly monitored in the reef coral Acropora hyacinthus during its reproductive cycle by using chloroform-methanol mixture extraction method and gas chromatography-mass spectrometry. The results are summarized here. The lipid content and tissues of the reef coral A. hyacinthus fluctuated markedly during the reproductive process, with the lipid content decreased drastically by approximately 43.4%and the tissues reduced by about 19.6% after reproduction. However, there was no significant difference in symbiotic algae density during the breeding process, implying that the eggs were probably without zooxanthellae. The reef coral A. hyacinthus accumulated lipid in the process of reproduction preparing. EPA (C20:5n-3, eicosapntemacnioc acid) and DHA (C22:6n-3, docosahexaenoic acid) played important roles in reproduction, since their contents changed significantly during this process. EPA and DHA increased by 41.3%and 36.5%, respectively, before breeding. However, their contents decreased by 32.0% and 34.3%, respectively, after breeding. Meanwhile, the content of poly-unsaturated fatty acids (PUFAs) also reduced appreciably, suggesting that they were intimately bound up with the reproduction. Scleractinian corals likely increased feeding to meet the need of fatty acids and energy during the reproductive process.