安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
1期
103-106
,共4页
周东浩%张建丽%刘红艳%陈淑红%刘桂娟%单晓阳%周爱金%彭焱%房辉%张向阳
週東浩%張建麗%劉紅豔%陳淑紅%劉桂娟%單曉暘%週愛金%彭焱%房輝%張嚮暘
주동호%장건려%류홍염%진숙홍%류계연%단효양%주애금%팽염%방휘%장향양
脑源性神经营养因子%2型糖尿病%认知障碍%单核苷酸多态性
腦源性神經營養因子%2型糖尿病%認知障礙%單覈苷痠多態性
뇌원성신경영양인자%2형당뇨병%인지장애%단핵감산다태성
brain-derived neurotrophic factor%type 2 diabetes%cognitive impairment%SNP
目的探讨脑源性神经营养因子( BDNF) rs12273539基因多态性和2型糖尿病认知障碍( T2DM-CI)之间的关联。方法选择 T2DM-CI 患者92例,无认知障碍的 T2DM (T2DM-NCI)患者191例,聚合酶链式反应-限制性片段长度多态性( PCR-RFLP )法检测 BDNF rs12273539基因多态性,SPSS 15.0及在线遗传软件SHEsis进行统计分析。结果在总体样本和男性样本中,BDNF rs12273539 TT基因型在认知障碍病例组分布相对过多,可能是T2DM-CI的易感基因型,在女性样本中BDNF-rs12273539等位基因和基因型分布在T2DM-CI组和T2DM-NCI组的差异无统计学意义。结论 BDNF-rs12273539可能是T2DM-CI的易感基因；BDNF-rs12273539基因多态性与T2DM-CI的关联存在性别差异。
目的探討腦源性神經營養因子( BDNF) rs12273539基因多態性和2型糖尿病認知障礙( T2DM-CI)之間的關聯。方法選擇 T2DM-CI 患者92例,無認知障礙的 T2DM (T2DM-NCI)患者191例,聚閤酶鏈式反應-限製性片段長度多態性( PCR-RFLP )法檢測 BDNF rs12273539基因多態性,SPSS 15.0及在線遺傳軟件SHEsis進行統計分析。結果在總體樣本和男性樣本中,BDNF rs12273539 TT基因型在認知障礙病例組分佈相對過多,可能是T2DM-CI的易感基因型,在女性樣本中BDNF-rs12273539等位基因和基因型分佈在T2DM-CI組和T2DM-NCI組的差異無統計學意義。結論 BDNF-rs12273539可能是T2DM-CI的易感基因；BDNF-rs12273539基因多態性與T2DM-CI的關聯存在性彆差異。
목적탐토뇌원성신경영양인자( BDNF) rs12273539기인다태성화2형당뇨병인지장애( T2DM-CI)지간적관련。방법선택 T2DM-CI 환자92례,무인지장애적 T2DM (T2DM-NCI)환자191례,취합매련식반응-한제성편단장도다태성( PCR-RFLP )법검측 BDNF rs12273539기인다태성,SPSS 15.0급재선유전연건SHEsis진행통계분석。결과재총체양본화남성양본중,BDNF rs12273539 TT기인형재인지장애병례조분포상대과다,가능시T2DM-CI적역감기인형,재녀성양본중BDNF-rs12273539등위기인화기인형분포재T2DM-CI조화T2DM-NCI조적차이무통계학의의。결론 BDNF-rs12273539가능시T2DM-CI적역감기인；BDNF-rs12273539기인다태성여T2DM-CI적관련존재성별차이。
Objective To investigate the genetic associations between polymorphisms of BDNF-rs12273539 and cognitive impairment in type 2 diabetes ( T2DM) patients. Methods Participants were divided into two groups:T2 DM patients complicated with cognitive impairment ( T2 DM-CI ) group ( n =92 ) and type 2 diabetes patients without cognitive impairment (T2DM-NCI) group (n=191). Polymorphic SNPs of rs12273539 in the BDNF gene were genotyped using PCR-RFLP technology. The description of data, case-control analysis and association analysis between SNPs were performed with the SPSS 15. 0 program and online SHEsis program. Results In the total sam-ples and male samples, there was also a significant difference in BDNF rs12273539 genotype distribution between the patients and control groups (X2 =7.639 4, P=0.021 9;X2 =6.643 0, P=0.036 1, respectively). In the fe-male samples, no significant association with T2DM-CI was observed between two groups (P>0. 05). Conclusion According to the present results, it can be concluded that:①the genetic polymorphisms of BDNF gene are like-ly to confer susceptibility to T2 DM-CI;②there are BDNF gene sex-specific genetic components involved in the pa-thology of T2DM-CI.
