江西林业科技
江西林業科技
강서임업과기
JIANGXI FORESTRY SCIENCE AND TECHNOLOGY
2014年
1期
1-5,9
,共6页
油茶%氧化鲨烯环化酶%β-香树素合成酶%羊毛甾醇合成酶
油茶%氧化鯊烯環化酶%β-香樹素閤成酶%羊毛甾醇閤成酶
유다%양화사희배화매%β-향수소합성매%양모치순합성매
Camellia oleifera%oxidosqualene cyclase%β-amyrin synthase%lanosterol synthas
氧化鲨烯环化酶（oxidosqualene cyclase，OSC）是甾醇和三萜合成的关键酶之一。以油茶(Camellia oleifera)未成熟种仁为材料进行转录组分析，并鉴定出4个OSC基因，分别命名为CoOSC1/CoCAS、CoOSC2/CobAS、CoOSC3/CoLAS及CoOSC4/CoaAS，CoOSC1和CoOSC2为全长序列。 OSC系统进化树表明，CoOSC1为环阿屯醇合成酶、CoOSC2为β-香树素合成酶、CoOSC3为羊毛甾醇合成酶、CoOSC4为α-香树素合成酶。
氧化鯊烯環化酶（oxidosqualene cyclase，OSC）是甾醇和三萜閤成的關鍵酶之一。以油茶(Camellia oleifera)未成熟種仁為材料進行轉錄組分析，併鑒定齣4箇OSC基因，分彆命名為CoOSC1/CoCAS、CoOSC2/CobAS、CoOSC3/CoLAS及CoOSC4/CoaAS，CoOSC1和CoOSC2為全長序列。 OSC繫統進化樹錶明，CoOSC1為環阿屯醇閤成酶、CoOSC2為β-香樹素閤成酶、CoOSC3為羊毛甾醇閤成酶、CoOSC4為α-香樹素閤成酶。
양화사희배화매（oxidosqualene cyclase，OSC）시치순화삼첩합성적관건매지일。이유다(Camellia oleifera)미성숙충인위재료진행전록조분석，병감정출4개OSC기인，분별명명위CoOSC1/CoCAS、CoOSC2/CobAS、CoOSC3/CoLAS급CoOSC4/CoaAS，CoOSC1화CoOSC2위전장서렬。 OSC계통진화수표명，CoOSC1위배아둔순합성매、CoOSC2위β-향수소합성매、CoOSC3위양모치순합성매、CoOSC4위α-향수소합성매。
Oxidosqualene cyclase (OSC) was one of the key enzymes in triterpene and sterol metabolism. Transcriptome analysis was conducted with Camellia oleifera immature kernel. Four different OSCs were identified, which were named CoOSC1/CoCAS, CoOSC2/CobAS, CoOSC3/CoLAS and CoOSC4/CoaAS, respectively. Both CoOSC1 and CoOSC2 were full-length sequences. OSCs phylogenetic tree analysis revealed that CoOSC1 was cycloartenol synthas, CoOSC2 was β-amyrin synthas, CoOSC3 was lanosterol synthas, and CoOSC4 wasα-amyrin synthas.
