CAJ | 학술논문

目的研究核黄素联合紫外光进行血小板病毒灭活的安全性、有效性及对血小板保存中白细胞释放细胞因子的抑制作用；观察灭活后血小板体外各参数的变化。方法实验组：将人巨细胞病毒标准株( HCMV AD169)注入核黄素溶液，混匀后加入到单采血小板中，以一定辐照剂量的紫外光照射，检测照射前后病毒滴度和照射后不同时间细胞因子的变化，并观察体外血小板部分参数的变化。对照组：为相同来源新鲜单采血小板，同步检测其细胞因子的含量和血小板体外各参数。以植物凝集素( PHA)同步刺激两组血小板，用ELISA法检测细胞因子的含量。结果实验组经150μmol/L的核黄素结合辐照剂量为1500 mJ/cm2的紫外光(250<λ<350 nm)照射10 min 可有效灭活血小板中病毒；对照组细胞因子含量随着保存时间的延长而显著增加；实验组第3天和第5天的细胞因子含量相对于保存前(0 d)差异无统计学意义；而在同一保存时间对照组中细胞因子含量高于实验组(P<0.05)；实验组和对照组血小板悬液经过PHA同步刺激后：接受PHA刺激的对照组与未接受PHA刺激的对照组相比，细胞因子含量显著增加( P <0.05)；而接受PHA刺激的实验组与未接受PHA刺激的实验组相比，细胞因子含量无显著变化。结论核黄素结合紫外光照射可以有效灭活血小板中病毒，抑制血小板保存中白细胞释放细胞因子的能力，而单采血小板体外诸参数和阴性对照比较无明显差异。
목적연구핵황소연합자외광진행혈소판병독멸활적안전성、유효성급대혈소판보존중백세포석방세포인자적억제작용；관찰멸활후혈소판체외각삼수적변화。방법실험조：장인거세포병독표준주( HCMV AD169)주입핵황소용액，혼균후가입도단채혈소판중，이일정복조제량적자외광조사，검측조사전후병독적도화조사후불동시간세포인자적변화，병관찰체외혈소판부분삼수적변화。대조조：위상동래원신선단채혈소판，동보검측기세포인자적함량화혈소판체외각삼수。이식물응집소( PHA)동보자격량조혈소판，용ELISA법검측세포인자적함량。결과실험조경150μmol/L적핵황소결합복조제량위1500 mJ/cm2적자외광(250<λ<350 nm)조사10 min 가유효멸활혈소판중병독；대조조세포인자함량수착보존시간적연장이현저증가；실험조제3천화제5천적세포인자함량상대우보존전(0 d)차이무통계학의의；이재동일보존시간대조조중세포인자함량고우실험조(P<0.05)；실험조화대조조혈소판현액경과PHA동보자격후：접수PHA자격적대조조여미접수PHA자격적대조조상비，세포인자함량현저증가( P <0.05)；이접수PHA자격적실험조여미접수PHA자격적실험조상비，세포인자함량무현저변화。결론핵황소결합자외광조사가이유효멸활혈소판중병독，억제혈소판보존중백세포석방세포인자적능력，이단채혈소판체외제삼수화음성대조비교무명현차이。
Objective To research the safety and efficacy of riboflavin ultraviolet light joint inactivation of virus, and the inhibition of leukocytes derived cytokines in platelets preservation and transfusion and to observe the bio-chemical alterations of the platelets following the inactivation measures. Methods In experimental group human cytomegalovirus ( HCMV AD169 ) was injected into the platelets suspension in final concentration of 150μmol/L of riboflavin solution. After mixing, the sample was put into the apheresis platelets, followed by ultraviolet light steri-lization, and the viral titer was tested in platelet suspension. The production cytokines were detected by ELISA on 0, 3 and 5 d after irradiation, and the change of platelets parameter was observed. Negative control group was com-posed of fresh apheresis platelets from the same collection without any treatments, cytokines were synchronously de-tected. Two groups of platelets were subjected to phytohemagglutinin ( PHA) for synchronous stimulation. Results A dose of 1 500 mJ/cm2 ultraviolet light combined with riboflavin gave rise to a effective sterilization of virus in the platelet suspension. The expression of leukocyte-derived cytokines was noted slight increase in the control group with the extension of saving time post-treatment. In the experimental group No. 3, 5 d the cytokine content had no significant difference relative to (0 d) before saving, while at the same retention time the cytokines content in the control group was higher than that in the experimental group ( P<0.05 );the two groups of platelet suspension after PHA synchronous stimulation:compared to the control group without PHA stimulated, the level of cytokines was in-creased significantly (P<0.05);while with respect to the experimental group without PHA stimulated, the level of cytokines showed no significant change. Conclusion Riboflavin plus ultraviolet light treatment can significantly in-activate selected virus, inhibit the production residual leukocytes of PLT release cytokines during storage, and in vitro apheresis platelets' various parameters compared with negative controls show no significant difference.