CAJ | 학술논문

目的探讨骨髓间充质干细胞( MSCs)及其上清液对大鼠佐剂性关节炎( AA)的预防作用。方法培养扩增SD大鼠骨髓MSCs,收集MSCs及其上清液。将健康雄性SD大鼠右后足跖皮内一次性注射弗氏完全佐剂0.1 ml/只造模。造模后行MSCs及其上清液干预。造模前1 d、造模后7、14、21、28 d测量大鼠体重和后足爪体积,同时观察大鼠生存状况。干预后第28天,行四肢关节X线片及病理检查。结果 MSCs干预组和上清液干预组大鼠,原发性炎症和继发病变均不明显,关节X线片和病理检查均未见明显异常,与模型组大鼠相比,体重和足爪体积差异有统计学意义(P <0.05)。结论 MSCs及其上清液可有效干预弗氏完全佐剂诱导的大鼠AA的进展。
목적탐토골수간충질간세포( MSCs)급기상청액대대서좌제성관절염( AA)적예방작용。방법배양확증SD대서골수MSCs,수집MSCs급기상청액。장건강웅성SD대서우후족척피내일차성주사불씨완전좌제0.1 ml/지조모。조모후행MSCs급기상청액간예。조모전1 d、조모후7、14、21、28 d측량대서체중화후족조체적,동시관찰대서생존상황。간예후제28천,행사지관절X선편급병리검사。결과 MSCs간예조화상청액간예조대서,원발성염증화계발병변균불명현,관절X선편화병리검사균미견명현이상,여모형조대서상비,체중화족조체적차이유통계학의의(P <0.05)。결론 MSCs급기상청액가유효간예불씨완전좌제유도적대서AA적진전。
Objective To explore the effect of marrow mesenchymal stem cells ( MSCs) and MSCs supernatant to prevent adjuvant arthritis ( AA) in rats and its mechanisms. Methods MSCs which had been cultured and cloned with supernatant would be collected. Rat's right rear paw of the model group was injected intracutaneously with 0. 1 ml of Freund's complete adjuvant. After modeling, the rats were given MSCs and MSCs supernatant intervention. During the period, the rats' weights and hind paw volumes were measured on the 1st, 7th, 14th, 21st and 28th days respectively. And the rats' living conditions were observed. On the 28st day of the intervention, X-ray exami-nation and pathological examination of rats' limb joints were carried out. Results After using MSCs supernatant to intervene adjuvant arthritis, primary inflammation and secondary lesions were not obvious, and joint X-ray and pathological examination showed no obvious abnormalities. Compared with model group, the differences of rats'weight and paw volume had statistically significant ( P<0.05 ) . Conclusion The results indicate that MSCs and MSCs supernatant can intervene the progress of AA.