中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2014年
10期
6-7
,共2页
许沈南%李佳杰%卢旭生%张伟亮
許瀋南%李佳傑%盧旭生%張偉亮
허침남%리가걸%로욱생%장위량
巨细胞病毒感染%胆总管囊肿
巨細胞病毒感染%膽總管囊腫
거세포병독감염%담총관낭종
Cytomegalovirus infection%Choledochal cyst
目的:探索人巨细胞病毒感染与胆总管囊肿的相关性。方法使用人巨细胞病毒荧光定量PCR分别对25例胆总管囊肿和20例对照组患者肝脏组织进行检测,同时进行病理染色和人巨细胞病毒晚期蛋白免疫组化检测。结果对照组均为检测到阳性结果,实验组中,人巨细胞病毒阳性14例(阳性率56%)。免疫组化结果显示阳性蛋白多位于肝细胞、血管内皮细胞、炎症浸润细胞当中,胆管上皮细胞呈强阳性反应。结论胆总管囊肿与人巨细胞病毒感染之间存在密切相关性。
目的:探索人巨細胞病毒感染與膽總管囊腫的相關性。方法使用人巨細胞病毒熒光定量PCR分彆對25例膽總管囊腫和20例對照組患者肝髒組織進行檢測,同時進行病理染色和人巨細胞病毒晚期蛋白免疫組化檢測。結果對照組均為檢測到暘性結果,實驗組中,人巨細胞病毒暘性14例(暘性率56%)。免疫組化結果顯示暘性蛋白多位于肝細胞、血管內皮細胞、炎癥浸潤細胞噹中,膽管上皮細胞呈彊暘性反應。結論膽總管囊腫與人巨細胞病毒感染之間存在密切相關性。
목적:탐색인거세포병독감염여담총관낭종적상관성。방법사용인거세포병독형광정량PCR분별대25례담총관낭종화20례대조조환자간장조직진행검측,동시진행병리염색화인거세포병독만기단백면역조화검측。결과대조조균위검측도양성결과,실험조중,인거세포병독양성14례(양성솔56%)。면역조화결과현시양성단백다위우간세포、혈관내피세포、염증침윤세포당중,담관상피세포정강양성반응。결론담총관낭종여인거세포병독감염지간존재밀절상관성。
Objective To explore the relationship between human cytomegalovirus and choledochal cyst. Methods Fluorescent quantitative PCR (FQ-PCR) was used to detect the liver tissue of human cytomegaloviru in 25 cases of choledochal cyst and 20 cases of control group. Meanwhile, immunohistochemistry was used to detect the human cytomegalovirus late proteins PP65 with the paraffin section. Results Among 25 cases of choledochal cyst, 14 cases (56%) were found to be positive for HCMV, meanwhile, the immunohistochemistry result showed that PP65 was located in the cytoplasm of infected cells such as liver cells, vascular endothelial cells, bile duct epithelial cells and inflammatory infiltrating cells. Conclusion HCMV has a close relationship with choledochal cyst.