CAJ | 학술논문

目的观察重组人p53腺病毒(Ad-p53)对人乳腺癌细胞MCF-7细胞形态、细胞生长、细胞凋亡和p53蛋白表达的影响。方法观察细胞形态学变化；MTS比色法观察Ad-p53对体外培养MCF-7细胞生长的抑制作用；流式细胞仪检测Ad-p53作用于MCF-7细胞72小时后细胞凋亡率和细胞周期的变化；Ad-p53作用于MCF-7细胞72小时后,Western blot法检测p53蛋白表达的情况。结果倒置显微镜下细胞形态发生明显的变化；MTS法观察到Ad-p53对乳腺癌细胞的抑制作用明显,且有时间依赖性；流式细胞术检测结果显示, Ad-p53作用于MCF-7细胞后其增殖抑制率与对照组相比明显增加。通过Western blot证实外源p53基因能在MCF-7细胞表达。结论 Ad-p53可抑制人乳腺癌细胞MCF-7的生长,促进其凋亡,其机制可能通过p53依赖途径实现。
목적관찰중조인p53선병독(Ad-p53)대인유선암세포MCF-7세포형태、세포생장、세포조망화p53단백표체적영향。방법관찰세포형태학변화；MTS비색법관찰Ad-p53대체외배양MCF-7세포생장적억제작용；류식세포의검측Ad-p53작용우MCF-7세포72소시후세포조망솔화세포주기적변화；Ad-p53작용우MCF-7세포72소시후,Western blot법검측p53단백표체적정황。결과도치현미경하세포형태발생명현적변화；MTS법관찰도Ad-p53대유선암세포적억제작용명현,차유시간의뢰성；류식세포술검측결과현시, Ad-p53작용우MCF-7세포후기증식억제솔여대조조상비명현증가。통과Western blot증실외원p53기인능재MCF-7세포표체。결론 Ad-p53가억제인유선암세포MCF-7적생장,촉진기조망,기궤제가능통과p53의뢰도경실현。
Objective To investigate the effect of Ad-p53 on the expression of p53 gene and the growth,cell cycle and apoptosis of MCF-7 cells. Method The changes of human breast cancer cell line MCF-7 morphology were ob-served by an inverted microscopy. MTS was applied to explore the impacts of Ad-p53 on the proliferation of MCF-7. After cells were cultured with Ad-p53 for 72h,the apoptosis rate and cell cycle were analyzed by flow cytometry. Western blot was used to detect the protein expression of p53. Result Significant changes of cell morphology in MCF-7 cells were observed by an inverted microscope. Ad-p53 could inhibit the growth of MCF-7 cells with optimal dose and best time. Flow cytometry revealed that Ad-p53 could increase the cell proliferation inhibition rate,and there was significant difference between MCF-7 group and control group. Western blotting results indicated that the p53 protein expression level in MCF-7 cells did not increase obviously with Ad-p53. Conclusion These results suggest that Ad-p53 can inhibit the cell growth and induce the cell apoptosis in p53-independent manner.