中国医学装备
中國醫學裝備
중국의학장비
CHINA MEDICAL EQUIPMENT
2012年
4期
70-72
,共3页
金黄色葡萄球菌%红霉素%克林霉素%诱导耐药
金黃色葡萄毬菌%紅黴素%剋林黴素%誘導耐藥
금황색포도구균%홍매소%극림매소%유도내약
Staphylococcus aureus%Erythromycin%Clindamycin%Inducible resistance
目的：了解我院临床分离的金黄色葡萄球菌中红霉素和克林霉素的耐药情况以及检测克林霉素诱导型耐药的发生率。方法：按照2007年美国临床实验室标准化研究所（CLSI）推荐的纸片扩散法,检测耐甲氧西林金黄色葡萄球菌（MRSA）及金黄色葡萄球菌中红霉素和克林霉素的耐药性,并用双纸片法（即D试验）检测红霉素对克林霉素诱导性耐药。结果：金黄色葡萄球菌中MRSA占71.7%（297/414）,红霉素、克林霉素同时耐药菌株在耐甲氧西林金黄色葡萄球菌（MRSA）和甲氧西林敏感金黄色葡萄球菌（MSSA）中分别占60.9%（181/297）和52.9%（62/117）,对红霉素耐药而克林霉素敏感的MRSA和MSSA中,D试验阳性分别为62.7%和64.5%。D试验阳性占所测金黄色葡萄球菌的20.3%,占红霉素耐药而克林霉素敏感的金黄色葡萄球菌的63.2%。结论：临床微生物实验室应开展D试验,检测金黄色葡萄球菌中红霉素对克林霉素的诱导性耐药,可以指导临床医师合理选用大环内酯类、林克酰胺类抗生素。
目的：瞭解我院臨床分離的金黃色葡萄毬菌中紅黴素和剋林黴素的耐藥情況以及檢測剋林黴素誘導型耐藥的髮生率。方法：按照2007年美國臨床實驗室標準化研究所（CLSI）推薦的紙片擴散法,檢測耐甲氧西林金黃色葡萄毬菌（MRSA）及金黃色葡萄毬菌中紅黴素和剋林黴素的耐藥性,併用雙紙片法（即D試驗）檢測紅黴素對剋林黴素誘導性耐藥。結果：金黃色葡萄毬菌中MRSA佔71.7%（297/414）,紅黴素、剋林黴素同時耐藥菌株在耐甲氧西林金黃色葡萄毬菌（MRSA）和甲氧西林敏感金黃色葡萄毬菌（MSSA）中分彆佔60.9%（181/297）和52.9%（62/117）,對紅黴素耐藥而剋林黴素敏感的MRSA和MSSA中,D試驗暘性分彆為62.7%和64.5%。D試驗暘性佔所測金黃色葡萄毬菌的20.3%,佔紅黴素耐藥而剋林黴素敏感的金黃色葡萄毬菌的63.2%。結論：臨床微生物實驗室應開展D試驗,檢測金黃色葡萄毬菌中紅黴素對剋林黴素的誘導性耐藥,可以指導臨床醫師閤理選用大環內酯類、林剋酰胺類抗生素。
목적：료해아원림상분리적금황색포도구균중홍매소화극림매소적내약정황이급검측극림매소유도형내약적발생솔。방법：안조2007년미국림상실험실표준화연구소（CLSI）추천적지편확산법,검측내갑양서림금황색포도구균（MRSA）급금황색포도구균중홍매소화극림매소적내약성,병용쌍지편법（즉D시험）검측홍매소대극림매소유도성내약。결과：금황색포도구균중MRSA점71.7%（297/414）,홍매소、극림매소동시내약균주재내갑양서림금황색포도구균（MRSA）화갑양서림민감금황색포도구균（MSSA）중분별점60.9%（181/297）화52.9%（62/117）,대홍매소내약이극림매소민감적MRSA화MSSA중,D시험양성분별위62.7%화64.5%。D시험양성점소측금황색포도구균적20.3%,점홍매소내약이극림매소민감적금황색포도구균적63.2%。결론：림상미생물실험실응개전D시험,검측금황색포도구균중홍매소대극림매소적유도성내약,가이지도림상의사합리선용대배내지류、림극선알류항생소。
Objective：To investigate the resistance of Staphylococcus aureus to clindamycin and erythromycin and detect the incidence of the inducible clindamycin resistance.Methods：We used the disk diffusion method（CLSI 2007）,to detect the resistance of MRSA and staphylococcus aureus to clindamycin and erythromycin.The double-disk diffusion method（D-test） was employed to detect erythromycin-induced clindamycin resistance.Results：MRSA was 71.7%（297/414） in Staphylococcus aureus,the co-resistance stains to erythromycin and clindamycin was 60.9%（181/297）in MRSA and 52.9%（62/117）in MSSA,resistance to erythromycin and clindamycin-sensitive in MSSA and MRSA,the positive rate of D-test was 62.7% and 64.5%.The positive rate of D-test was 20.3% in Staphylococcus aureus,D-test positive stains was 63.2% in resistance to erythromycin and clindamycin-sensitive strains.Conclusion：D-test should be implemented in clinical microbiological laboratory.To detect the Erythromycininduced resistance to clindamycin in Staphylococcus aureus can guide clinicians to rationally prescribe macrolides and link amide antibiotics to patients.
