CAJ | 학술논문

本文建立了水产品中10种大环内酯类抗生素的高效液相色谱-电喷雾串联质谱检测方法。对比几种检测方法后，最终采用乙腈进行提取的方法。在5．0-200．0μg／L范围内，10种大环内酯类抗生素的线性相关系数均大于0．99。通过实际样品的添加回收试验，方法的定量下限（S／N=10）为1．0μg／kg，检出限（S／N=3）为0．2μg／kg，3个添加水平中，龙虾、鲴鱼的加标回收率分别为69．4％-115．3％和63．1％-100．9％，日内相对标准偏差（RSD）分别为0．73％-3．39％及0．8％-4．44％。结果表明，该法简单、灵敏、特异性强，适用于水产品中大环内酯类药物残留的分析确证。
본문건립료수산품중10충대배내지류항생소적고효액상색보-전분무천련질보검측방법。대비궤충검측방법후，최종채용을정진행제취적방법。재5．0-200．0μg／L범위내，10충대배내지류항생소적선성상관계수균대우0．99。통과실제양품적첨가회수시험，방법적정량하한（S／N=10）위1．0μg／kg，검출한（S／N=3）위0．2μg／kg，3개첨가수평중，룡하、고어적가표회수솔분별위69．4％-115．3％화63．1％-100．9％，일내상대표준편차（RSD）분별위0．73％-3．39％급0．8％-4．44％。결과표명，해법간단、령민、특이성강，괄용우수산품중대배내지류약물잔류적분석학증。
Determination of 10 kinds of Macrolide durgs by high performance liquid chromatography tandem mass spectrometry （ HPLC - MS/MS） in aquatic products has been studied in this research. Contrast several extraction methods, samples finally were extracted by acetonitrile. In the range of 5.0 - 200.0μg/L, the linear correlation coefficients for the determination for the above ten kinds of macrolide antibiotics were greater than 0.99. The detection quality （S/N = 10） and the detection limit （S/N = 3 ） were 1.0 μg/kg and 0.2 p,g/kg, respectively. Recoveries for the lobster, catfish of fish, honey and milk were 69.4% - 115.3 % ,63.1% to 100.9% , RSD in the day were 0.73% - 3.39% and 0. 8% - 4.44%. Results indicated that the established method is simple, sensitive, and specific for the determination of macrolide residues in aquatic products.