CAJ | 학술논문

采用高效液相色谱法，在（23．1±0．8）℃水温条件下，对环丙沙星和磺胺二甲嘧啶两种抗菌药物在健康大菱鲆体内的代谢动力学规律进行了比较研究。结果显示，单次口服环丙沙星和磺胺二甲嘧啶后，药物在血浆中的经时过程均符合一级吸收二室开放模型，表达方程分别为CIP=14．811e^-0.337t＋4．028^e-0.63t-18．839e^-0.616t、CSM2=64．981e^-0.141t＋4．59e^0.004r-69．571e^-0.19t；静脉注射这两种药物后，药物在血浆中经时过程均符合无级吸收二室开放模型，表达方程分别为CCIP=21．784e^-1.098t＋1．514e^-0.043t、CSM2=33．028e^-5.687t＋8．674e^-0.013t。口服相同剂量（20mg／kg）药物后，对血浆的药代动力学参数进行比较，环丙沙星的Tmax（6h）、Cmax（5．385μg／mL）、t1／2Ka（1．125h）、t1／2α（2．057h）和t1／2β（11．028h）均小于磺胺二甲嘧啶给药（8h、13．990μg／mL、3．647h、4．923h和173．407h），且F（60．57％）大于磺胺二甲嘧啶给药F（47．13％）。证实环丙沙星在大菱鲆体内的吸收、分布、消除速度，达峰时间均快于磺胺二甲嘧啶给药，且比磺胺二甲嘧啶给药吸收完全。根据本实验的结果，环丙沙星和磺胺二甲嘧啶的合理给药方案分别为28．01mg／kg和18．32mg／kg，均为每日1次给药，连用3-5d。
채용고효액상색보법，재（23．1±0．8）℃수온조건하，대배병사성화광알이갑밀정량충항균약물재건강대릉평체내적대사동역학규률진행료비교연구。결과현시，단차구복배병사성화광알이갑밀정후，약물재혈장중적경시과정균부합일급흡수이실개방모형，표체방정분별위CIP=14．811e^-0.337t＋4．028^e-0.63t-18．839e^-0.616t、CSM2=64．981e^-0.141t＋4．59e^0.004r-69．571e^-0.19t；정맥주사저량충약물후，약물재혈장중경시과정균부합무급흡수이실개방모형，표체방정분별위CCIP=21．784e^-1.098t＋1．514e^-0.043t、CSM2=33．028e^-5.687t＋8．674e^-0.013t。구복상동제량（20mg／kg）약물후，대혈장적약대동역학삼수진행비교，배병사성적Tmax（6h）、Cmax（5．385μg／mL）、t1／2Ka（1．125h）、t1／2α（2．057h）화t1／2β（11．028h）균소우광알이갑밀정급약（8h、13．990μg／mL、3．647h、4．923h화173．407h），차F（60．57％）대우광알이갑밀정급약F（47．13％）。증실배병사성재대릉평체내적흡수、분포、소제속도，체봉시간균쾌우광알이갑밀정급약，차비광알이갑밀정급약흡수완전。근거본실험적결과，배병사성화광알이갑밀정적합리급약방안분별위28．01mg／kg화18．32mg／kg，균위매일1차급약，련용3-5d。
The pharmacokinetics of ciprofloxacin and sulfamethazine was studied in Scophthalmus maximus by using the high performance liquid chromatography （HPLC）. The study was performed at（23.1 ±0.8 ）℃. All the health frish received a single oral administration of CIP and SM2 at a level of 20 mg/kg and intravenous administration of CIP and SM2 at a level of 10 mg/kg. The results showed that the hemolymph concentration - time course of CIP and SM2 can be described by a two - compartment open model with the first order absorption after a single oral administration, the pharmacokinetic equations were： CIP=14.811e^-0.337t＋4.028^e-0.63t-18.839e^-0.616t、CSM2=64.981e^-0.141t＋4.59e^0.004r-69.571e^-0.19t. The hemolymph concentration - time course of CIP and SM2 can be described by a two - compartment open model with the stepless absorption after a singleintravenous administration, the pharmacokinetic equations were ： CCIP=21.784e^-1.098t＋1.514e^-0.043t、CSM2=33.028e^-5.687t＋8.674e^-0.013t. After the same dose oral drugs, the main pharmacokinetics parameters in plasma were those： the peak time, peak concentration, absorption, distribution and elimination half- lives （Tmax, Cmax t1/2Ka ,t1/2α and t1/2β） by ciprofloxacin administration were found to be 6 h, 5. 385 p.g/mL, 1. 125 h, 2. 057 h and 11.028 h which were shorter than 8 h, 13. 990 μg/mL, 3. 647 h, 4. 923 h and 173. 407 h of sulfamethazine administration, but its bioavailability 60. 57% were bigger than sulfamethazine administration which was 47.13%. The results indicate that the peak time, absorption, distribution and eliminate speed of ciprofloxacin administration were faster than sulfamethazine administration, ciprofloxacin was absorbed completely. To the experimental results, the scheme of ciprofloxacin and sulfamethazine was established to fish bacterial diseases, with the dose of 28. 01 mg/kg and 18. 32 mg/kg at the interval of 1 d, continuous 3 -5 days by oral administration, respectively.