CAJ | 학술논문

液相阻断ELISA（LBE）法由于具有高度的敏感性、特异性和重复性,目前被广泛应用于动物免疫抗体效果监测实践工作中,特别是为防控牛、羊亚洲I型口蹄疫（FMD）做出了积极的贡献。但该方法操作繁琐,稍有不慎就会导致实验结果发生偏差或试验失败,笔者就该方法应用于亚I型口蹄疫抗体检测中的改进和体会作一总结。
액상조단ELISA（LBE）법유우구유고도적민감성、특이성화중복성,목전피엄범응용우동물면역항체효과감측실천공작중,특별시위방공우、양아주I형구제역（FMD）주출료적겁적공헌。단해방법조작번쇄,초유불신취회도치실험결과발생편차혹시험실패,필자취해방법응용우아I형구제역항체검측중적개진화체회작일총결。
As its high sensitivity, specificity and reproducibility, liquid blocking ELISA （ LBE ） method has been widely used m testing and monitoring of animal immune antibody in practice, and has made great contribution to prevention and control of foot-and- mouth disease （FMD） of cattle and sheep. But the method has fussy operation, and slight carelessness will lead to deviation or failure of experimental results, the authors make a summary of improvement of the method and its application to FMD antibody detection in experience.