CAJ | 학술논문

目前检测卵黄抗体的方法很多,但IHA方法具有简便快速的优点,本研究的目的是为了建立抗犬瘟热高免卵黄抗体检测方法。采用犬瘟热病毒和犬细小病毒二联疫苗处理作为疫苗抗原,用犬瘟热病死犬的肝脏部分处理后作为组织抗原,制备敏化绵羊红细胞,用IHA方法确定了犬二联疫苗抗原和犬瘟热病毒组织抗原的最适含量。二联疫苗提取抗原的含量为0.7 mg/mL致敏红细胞时,与犬瘟热单克隆抗体反应显著,抗体效价为1∶256;而患犬瘟热松狮犬的肝脏组织抗原蛋白浓度为0.5mg/mL,与犬瘟热单克隆抗体反应显著,抗体效价为1∶128。经过IHA验证,制备的松狮犬瘟热肝组织抗原,蛋白含量0.5 mg/mL 1%致敏红细胞,对应的单抗、鸡血清均出现了凝集,阳性对照也出现凝集,阴性对照未出现凝集。建立的IHA抗体检测方法,为准确检测制备抗犬瘟热高免卵黄抗体的效价奠定了基础。
목전검측란황항체적방법흔다,단IHA방법구유간편쾌속적우점,본연구적목적시위료건립항견온열고면란황항체검측방법。채용견온열병독화견세소병독이련역묘처리작위역묘항원,용견온열병사견적간장부분처리후작위조직항원,제비민화면양홍세포,용IHA방법학정료견이련역묘항원화견온열병독조직항원적최괄함량。이련역묘제취항원적함량위0.7 mg/mL치민홍세포시,여견온열단극륭항체반응현저,항체효개위1∶256;이환견온열송사견적간장조직항원단백농도위0.5mg/mL,여견온열단극륭항체반응현저,항체효개위1∶128。경과IHA험증,제비적송사견온열간조직항원,단백함량0.5 mg/mL 1%치민홍세포,대응적단항、계혈청균출현료응집,양성대조야출현응집,음성대조미출현응집。건립적IHA항체검측방법,위준학검측제비항견온열고면란황항체적효개전정료기출。
The purpose of this study was to establish a method to detect hyperimmunized immunoglobuin of Yolk of canine distemper virus. Canine distemper virus and canine parvovirus treatment bivalent vaccine was used as the vaccine antigen, and the liver of a dead canine caused by canine distemper virus was treated and used for tissue antigen. The sensitized sheep red blood cells were prepared, The IHA method was applied to determine the optimal levels of canine bivalent vaccine antigen abd distemper virus tissue antigens. When extraction of bivalent vaccine antigen content was 0.7 mg / mL sensitized red blood cells, the monoclonal antibody reacted with canine distemper significantly, and the antibody titer was 1：256;The liver tissue antigen concentration of Chow Chow dogs suffering from distemper was 0.5 mg / mL, the monoclonal antibody reacted with canine distemper significantly, and the antibody titer was l：128.After IHA verification, protein content was 0.5 mg/ roLl% sensitized red blood cells in liver tissue of antigen Chow suffering from distemper,the corresponding monoclonal antibody and chicken serum agglutination occured, positive control agglutination also occured, agglutination negative control did not appear . The establishment of an IHA method can provide a basis for detecting hyperimmunized immunoglobuin of Yolk of canine distemper virus.