河北化工
河北化工
하북화공
HEBEI CHEMICAL ENGINEERING AND INDUSTRY
2012年
4期
14-16,19
,共4页
Ppd%肺癌A549细胞%Hochest%33258
Ppd%肺癌A549細胞%Hochest%33258
Ppd%폐암A549세포%Hochest%33258
20(S)-protopanaxadiol(Ppd)%lung cancer A549 cell%Hochest 33258
采用台盼蓝和MTT法测定20（S）-原人参二醇（Ppd）对肺癌A549细胞增殖的抑制作用,Hochest 33258染色和DNA Ladder方法观察Ppd对小细胞肺癌A549细胞凋亡及周期的影响。结果表明,Ppd对肺癌A549细胞具有明显的抑制作用并有剂量时间依赖关系,可明显降低肺癌A549细胞的存活率和活性（IC50为20.7μmol/L）;染色后肺癌A549细胞核出现典型的凋亡形态学变化,与DNA Ladder电泳条带弥散情况一致。因此,Ppd对肺癌A549细胞的增殖有明显的抑制作用。
採用檯盼藍和MTT法測定20（S）-原人參二醇（Ppd）對肺癌A549細胞增殖的抑製作用,Hochest 33258染色和DNA Ladder方法觀察Ppd對小細胞肺癌A549細胞凋亡及週期的影響。結果錶明,Ppd對肺癌A549細胞具有明顯的抑製作用併有劑量時間依賴關繫,可明顯降低肺癌A549細胞的存活率和活性（IC50為20.7μmol/L）;染色後肺癌A549細胞覈齣現典型的凋亡形態學變化,與DNA Ladder電泳條帶瀰散情況一緻。因此,Ppd對肺癌A549細胞的增殖有明顯的抑製作用。
채용태반람화MTT법측정20（S）-원인삼이순（Ppd）대폐암A549세포증식적억제작용,Hochest 33258염색화DNA Ladder방법관찰Ppd대소세포폐암A549세포조망급주기적영향。결과표명,Ppd대폐암A549세포구유명현적억제작용병유제량시간의뢰관계,가명현강저폐암A549세포적존활솔화활성（IC50위20.7μmol/L）;염색후폐암A549세포핵출현전형적조망형태학변화,여DNA Ladder전영조대미산정황일치。인차,Ppd대폐암A549세포적증식유명현적억제작용。
To study the effects of 20（S）-protopanaxadiol （Ppd）on the proliferation of human lung carcinoma A549 cell line. Human lung carcinoma A549 cell was treated with Ppd ,inhibition of Ppd on proliferation of lung cancer A549 cells was determined with trypan blne and MTT, cell grow thrate was determined with MTT assay. The effects of Ppd on A549 cell and cell cycle were observed by the flow cytometer. Results showed that Ppd could inhibit the prolife ration of human lung carcinoma A549 cell in atime and dose dependent manner. Ppd inhibited the growth of human lung carcino- ma A549 cells when IC50 was 20.7μmol/L. The morphological changes were observed in the nuclei of human lung carcinoma A549 cells treated by Ppd. The DNA Ladders bands were detected.Therefore,Ppd could inhibit the proliferation of lung cancer A549 cell.
