CAJ | 학술논문

以RP59500为试验菌株,在15 L发酵罐内,研究了普那霉素的发酵工艺。通过单因素条件试验确定了其生物合成的最适温度、接种量。优化了其发酵培养基,并进行了发酵中间补料试验,确定了最佳补料工艺。试验结果表明：以3%淀粉、1.7%蔗糖、1%黄豆粉、1.5%酵母粉、0.5%葡萄糖、0.01%KH2PO4、0.2%MgSO4、0.2%泡敌和0.4%CaCO3的发酵培养基配方,以接种量4.0%,26℃下发酵,在发酵21 h时补加氨基酸,普那霉素的发酵效价达3 000 U/mL。
이RP59500위시험균주,재15 L발효관내,연구료보나매소적발효공예。통과단인소조건시험학정료기생물합성적최괄온도、접충량。우화료기발효배양기,병진행료발효중간보료시험,학정료최가보료공예。시험결과표명：이3%정분、1.7%자당、1%황두분、1.5%효모분、0.5%포도당、0.01%KH2PO4、0.2%MgSO4、0.2%포활화0.4%CaCO3적발효배양기배방,이접충량4.0%,26℃하발효,재발효21 h시보가안기산,보나매소적발효효개체3 000 U/mL。
The fermentation techology of pristinamycin with RP59500 as experimental strains was studied in a 15 L fermentor. Through single-factor experiments, the optimum fermentation temperature, inoculation volume were obtained. The fermentation medium composition was optimized.The titer of pristinamycin increased greatly by using feeding fermentation. The results showed that the titer of pristinamycin reached 3 000 μ/mL with 4.0 % inoculation volume at 26 ℃. The optimal medium contains 3% starch, 1.7% sucrose, 1% soybean powder, 1.5% yeast powder, 0.5% glucose, 0.01% KH2PO4,0.2% MgSO4, 0.2% defoaming agent, 0.4% CaCO3, and the amino acid was fed after it had been fermentated for 21 h.