CAJ | 학술논문

[目的]对猪MSTN基因其进行生物信息学分析。[方法]以从GenBank中检索到的猪、大鼠、小鼠、狗、绵羊、山羊、牛、黑猩猩、人、马、鸡和斑马鱼的MSTN基因CDS序列为材料，将该12个物种的MSTN蛋白序列调人DNAStar软件的Megalign程序，进行系统进化分析；并对猪MSTN基因的基本信息、内切酶图谱、编码蛋8--级结构、信号肽、跨膜结构以及蛋白质亚细胞定位进行分析。[结果]猪MSTN基因与大鼠、小鼠、狗、绵羊、山羊、牛、黑猩猩、人、马亲缘关系很近；该基因包含多个酶切位点；其编码的蛋白是一个疏水性不稳定蛋白，分子量为42791．3U，等电点为6．98，包含375个氨基酸残基；蛋白二级结构上，含有20．53％的α-螺旋（Helix）、4％β-转角（Turn）、55．07％无规则卷曲（Coil）、22．4％伸展条（extendenStrand）和1个跨膜结构区域；该蛋白定位于细胞外，作为信号肽的可能性很大。[结论]该研究为猪MSTN基因的进一步分析研究提供了参考依据。
[목적]대저MSTN기인기진행생물신식학분석。[방법]이종GenBank중검색도적저、대서、소서、구、면양、산양、우、흑성성、인、마、계화반마어적MSTN기인CDS서렬위재료，장해12개물충적MSTN단백서렬조인DNAStar연건적Megalign정서，진행계통진화분석；병대저MSTN기인적기본신식、내절매도보、편마단8--급결구、신호태、과막결구이급단백질아세포정위진행분석。[결과]저MSTN기인여대서、소서、구、면양、산양、우、흑성성、인、마친연관계흔근；해기인포함다개매절위점；기편마적단백시일개소수성불은정단백，분자량위42791．3U，등전점위6．98，포함375개안기산잔기；단백이급결구상，함유20．53％적α-라선（Helix）、4％β-전각（Turn）、55．07％무규칙권곡（Coil）、22．4％신전조（extendenStrand）화1개과막결구구역；해단백정위우세포외，작위신호태적가능성흔대。[결론]해연구위저MSTN기인적진일보분석연구제공료삼고의거。
[Objective] The aim was to make bioinformatics analysis on MSTN gene in swine. [Method] In the research, coding sequences （CDS） of MSTN gene in swine, rat, mouse, dog, sheep, goat, cattle, chimpanzee, human, horse, chicken and zebra fish were loaded to Megalign of DNAstar for analysis of phyletic evolution. In addi- tion, we also made analysis on basic information, restriction map, secondary struc- ture of coding protein, signal peptide, transmembrane domain and protein subcellular localization. [Result] Closely related to rat, mouse, dog, sheep, goat, cattle, chim- panzee, human and horse, MSTN genes in swine included many enzyme cutting sites and the encoding protein was unstable in hydrophobicity. The molecular weight was 42 791.3 u, isoelectric point was 6.98, and the gene included 375 amino acid residues. In addition, the secondary structure of protein contained 20.53% of c~-Helix, 4% of 15-Turn, 53.07% of Coil, 22.4% of extenden strand and one transmembrane domain. The extracellular protein was probably being signal peptide. [Conclusion] The research provided references for further study on MSTN genes of swine.