CAJ | 학술논문

[目的]采用叶绿素成像技术测定细叶榕（FicusmicrocarpaL．）叶片对聚乙二醇模拟的水分胁迫的响应。[方法]人工模拟水分胁迫下检测细叶榕、垂叶榕和极端耐旱的欧洲夹竹桃离体叶片，并用叶绿素荧光成像技术同时提取单片叶多个部位进行检测和分析。[结果]细叶榕、垂叶榕和夹竹桃对脱水响应的结果表明：①叶片各测定部位之间的光合反应中心最大光能转换效率（n／m）和非光化学猝灭（NPQ）的值较小，而电子传递速率（ETR）、PSII真实光化效率（ΦPSD）、光化学猝灭（qP）则有较大的差异；②水分胁迫下叶片不同部位的这种离散性更加明显；③抗逆性强的离散程度明显变小。[结论]为叶绿索成像技术进一步应用于植物对干旱胁迫的响应研究奠定了基础。
[목적]채용협록소성상기술측정세협용（FicusmicrocarpaL．）협편대취을이순모의적수분협박적향응。[방법]인공모의수분협박하검측세협용、수협용화겁단내한적구주협죽도리체협편，병용협록소형광성상기술동시제취단편협다개부위진행검측화분석。[결과]세협용、수협용화협죽도대탈수향응적결과표명：①협편각측정부위지간적광합반응중심최대광능전환효솔（n／m）화비광화학졸멸（NPQ）적치교소，이전자전체속솔（ETR）、PSII진실광화효솔（ΦPSD）、광화학졸멸（qP）칙유교대적차이；②수분협박하협편불동부위적저충리산성경가명현；③항역성강적리산정도명현변소。[결론]위협록색성상기술진일보응용우식물대간한협박적향응연구전정료기출。
[Objective] This study was to determine the response of Ficus microcarpa L. foliage to polyethylene glycol （PEG） simulated water stress using chlorophyll fluo- rescence imaging technique. [Method] The responses of detached leaves from Ficus microcarpa, Ficus benjamina and Nerium oleander to PEG-6000 simulated water stress were detected, and the chlorophyll fluorescence imaging technique was used to detect and analyze the stress at different spots of a single leaf simultaneously. [Result] The responses of Ficus microcarpa, Ficus benjamina and Nerium oleander to dehydration showed that： ~1~） the maximal photochemical efficiency （Fv/Fm） and non- photo-chemical quenching （NPQ） values were small in the reaction center among different detected spots of leaves, and there were great differences between relative electron transport rate （ETR）, photochemical quenching （qP） and quantum efficiency of PSII photochemistry （（φPSII）; （2） the differences of these parameters were more ob- vious among different spots of water-stressed leaves; （3） the discrete degrees of the species with strong resitances decreased significantly. [Conclusion] This study lays the foundation for the further research on the response of plants to drought stress using chlorophyll fluorescence imaging technique.