CAJ | 학술논문

[目的]介绍一个从马鹿粪便中提取DNA的改进方法。[方法]本实验采用的是，在传统的CTAB裂解法的基础下，根据马鹿粪便的特征摸索出来的改进方法。[结果]采用该方法从天山马鹿粪便中提取了高质量的粪便DNA并扩增了天山马鹿线粒体细胞色素b基因片段，通过测序检测，同时提取马鹿肌肉和皮毛样品的DNA作为对照，进一步证实了提取的可靠性。[结论]该方法提取过程中不需要用蛋白酶K；所提取的DNA不需要州DNA纯化试剂盒纯化，可直接用PCR扩增，因此，费用量很低。
[목적]개소일개종마록분편중제취DNA적개진방법。[방법]본실험채용적시，재전통적CTAB렬해법적기출하，근거마록분편적특정모색출래적개진방법。[결과]채용해방법종천산마록분편중제취료고질량적분편DNA병확증료천산마록선립체세포색소b기인편단，통과측서검측，동시제취마록기육화피모양품적DNA작위대조，진일보증실료제취적가고성。[결론]해방법제취과정중불수요용단백매K；소제취적DNA불수요주DNA순화시제합순화，가직접용PCR확증，인차，비용량흔저。
[Objective] To introduce an improved method for DNA extraction from the faeces of red deer. [Method] Based on the traditional method of CTAB lysis, we proposed an improved DNA extraction method according to the characteristics of red deer faeces. [Result] This improved method extracted high-quality fecal DNA from Tianshan red deer and amplified the mitochondrial cytochrome b gene. With the muscle and fur DNA of red deer as the control, the sequencing results further con- firmed the reliability of the method. [Conclusion] The method requires no proteinase K in the process of extraction, and the extracted DNA can be used for PCR ampli- fication directly without the purification of DNA purification kit, thus, it is cost-saving.